Monthly Archives: October 2013

Autoclaves Precautions For Use

Autoclaves Precautions For Use

1 Sterilizer disinfection should be used indoors, operating by professional, shall not be used in public places.

2 Outlet must be fitted with ground connection, be sure that the power plug is inserted firmly. Do not damage the wires or use unspecified power cord. Connect the power cord on the ground to protect the springs, should keep good touch.

3 Safety valve and pressure gauge should be sent to identification of legal metrology inspection department after a full year lifespan.

4 Keep a gap between items, sequentially stacked in the sterilizer sieve, it is a good steam penetration, can improve the sterilization effect.

5 Pay attention to the power cord should be plugged tightly when heating, copper grounding the plug in close contact with the shield, to ensure safe use. The start of heating steam valve will be placed on the vertical open position pick, sterilizer cold air will be heated and overflow by the valve hole at any time. When the valve hole with sharp steam out , it will pick the valve back into place . Works disinfection desired pressure rang , the appropriate adjustment or off heat , so that the maintenance of constant pressure and start to calculate disinfection time , according to different items and packaging in the required sterilization time .

6 Dressings, disinfecting equipment and utensils required after drying , it would be the end of the sterilizer disinfecting the steam discharged from the discharge steam valve when the pressure gauge pointer back to zero wait 1-2 minutes after the lid open , and heating continued for a few minutes , so that items can achieve the purpose of drying .

7 Disinfecting solution medium, etc. If release steam immediately at the end, due to a sudden drop in pressure caused by blasting or bottle sterilizer interior solution overflows and other serious accidents. So in the end should not release disinfecting steam immediately, but should stop heating so that naturally cooled 20-30 minutes, the pressure inside the cooling dropped to zero (press service table pointer back to zero ) after a few minutes , put the steam valve open, and then open the lid slightly ( to open a gap ) , leave sterilization room , wait until the natural cooling to a certain extent and then take it out.

More details, Please contact Bluestone Industrial & Trading Ltd. and view http://www.autoclavesale.com/ website

Monitoring Methods of High-pressure Steam Sterilization Effect

Monitoring Methods of High-pressure Steam Sterilization Effect

The effect of high-pressure steam sterilization is how to be monitored out, need to have some monitoring indicators , the monitoring of high-pressure steam sterilization effect mainly have the following three ways :

First is the process monitoring , procedures monitoring. Depending on the installing sterilizer gauges ( pressure gauges, thermometers , Chronograph ) , charts, indicating needle , alarms , etc., indicate sterilization equipment is working properly or not. This method can be quick to point out sterilizer failure , but can not determine whether the items to reach sterilization standard . This method as a routine monitoring method, should be carried out every time when sterilizing.

Second is the chemical indicator monitoring . Using the discoloration or deformation feature under heat of chemical indicator in a certain temperature and reaction time, to determine whether reached the sterilization parameters.

Commonly used are : 1 ) self- temperature tube: Make crystals of certain chemicals sealed in a glass tube ( length 2cm, diameter of 1 ~ 2mm). Common reagent like benzoic acid ( melting point 121-123 ℃) and so on. Sterilizing , when the humidity rises to the melting point of the drug, the crystal in tube will be melted, although \ cooled and then solidified , its shape is still different from original shape, this method can only indicate the temperature , does not indicate whether the duration of heat have been met , and therefore minimum standards. Mainly used for monitoring  the packaging center of all kinds of goods .

2) 3M pressure sterilization indicator tape : This tape printed with instructions oblique white line pattern , is a sterile affixed outside of the package to be sterilized in a special color tape . Paste surface dressing can be firmly closed package , metal box or glass article of 20 minutes at 121 ℃ , 130 ℃ for 4 minutes after the tape 100% color ( black stripe pattern that appears diagonal stripes ) . 3M tape both for monitoring goods packaging surface, but also for monitoring of the packaging center , you can also replace the pins, clips or tape to use .

Third is a biological indicator monitoring . Using heat-resistant non-pathogenic bacterial spores as indicator bacteria to determine the effect of heat sterilization . Bacillus stearothermophilus strains with the bacteria Bacillus strong resistance to heat , the thermal death time and the strongest resistance to pathogenic microorganisms similar botulinum spores . Biological indicators are spore suspension Bacillus bacteria plate and sheet, and mixed medium of instruction pipe . Testing should use standard test packets, each packet center position biological indicator 2 , on the sterilizer chamber 5 points , namely , the middle point of the center of each one , the lower the before, during and after a point . After sterilization , take out the biological indicator , bromocresol purple inoculated glucose peptone water medium , set 55-60 ℃ incubator for 48 hours to 7 days observations final result. If the color unchanged after culturing , clear and transparent , indicating that spores have been killed . Reached the sterilization requirements . If turns yellow turbid , means spores have not been killed , sterilization failed.

More details, Please contact Bluestone Industrial & Trading Ltd. and view http://www.autoclavesale.com/ website

Talk About The High Pressure Sterilizer Operation Steps

Talk About The High Pressure Sterilizer Operation Steps

1 . First, take out the inner basket, and then add appropriate amount of water into the outer pot, so the water level is appropriate phase with the triangular shelf .

2 . Back into basket and load items to be sterilized . Be careful not to install too crowded, so as not to impede steam circulation and affect sterilization effect. Erlenmeyer flasks and test tube ends are not in contact with the tank wall to avoid condensation wet paper bag mouth penetration tampon .

3 . Cover the basket , and insert the cover of the exhaust hose into the exhaust slot of inner basket barrel. Then twenty-two relatively symmetrical manner while tightening the two bolts , the bolt tightness consistent , must not allow leakage.

4 . With electric or gas heating , and also open the exhaust valve , so that the pot of boiling water to eliminate the cold air . When the cold air is exhausted , close the exhaust valve , so that the temperature of the pot with the steam pressure increases gradually. When the pressure is raised to the desired pressure pot , control heating, maintaining pressure to the desired time . In this experiment 1.05kg/cm2, 121.3 ℃, 20minutes sterilization.

5 . After sterilization, cut off the power supply or turn off the gas , so that the pot temperature sterilization drop naturally , when the pressure gauge drop to 0 , open the exhaust valve, unscrew the bolt and open the lid , take out the sterilized items. If the pressure is not reduced to 0 when the exhaust valve opens , a sudden pressure drop will be due to the pot , the medium within the container and the external pressure imbalance out port or tube mouth flask , resulting in the occurrence of the tampon contaminated medium contamination.

6 . Put the medium into sterile 37 ℃ incubator for 24 hours , after inspecting without bacteria growth , all set.

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High-pressure Steam Sterilization Precautions

High-pressure Steam Sterilization Precautions

High-pressure steam sterilization method is a rapid and effective method of sterilization . High-pressure steam sterilizer , generate steam by heating , with increasing steam pressure , a rise in temperature , typically the pressure 103.4kPa ( rather old system 15 lb / inch 2 or 1.05kg/cm 2) , the inside temperature up to 121.3 ℃, keep  15 ~ 30min, can kill all microorganisms , including spores , including . This method is commonly used in the general saline , surgical instruments and dressings and other goods humidity and high temperature sterilization .

High-pressure steam sterilization precautions :
First, the sterile package is not too large ( less than 50cm × 30cm × 30cm), should not be too tight , have a gap between each parcel , so that the steam can easily penetrate into the parcel central convection . Before disinfection , open tanks or boxes vent steam circulation in favor . And quickly exhaust the steam is discharged to keep the dry goods . When sterilization is completed, close the vent tank or cartridge to maintain sterility of the goods .
Second , cloth items should be placed on metal objects , otherwise the steam condensed into water droplets freeze so damp cloth . Hinder steam into parcels central , seriously affecting the sterilization effect.
Third, check the inspection sterilization effect periodically. After autoclaving sterile packs , sterile container to one week period is appropriate. Monitoring the effect of high-pressure steam sterilization : There are following three methods .
The first is the process monitoring ,also known as procedures monitoring. Depending on the installation on the sterilizer gauges ( pressure gauges, thermometers , Chronograph ) , charts, indicating needle , alarms , etc., indicate sterilization equipment is working properly or not. This method can be quick to point out sterilizer malfunction, but can not determine whether the items reach the sterilized sterilization requirements . This method as a routine monitoring methods , sterilization should be carried out every time .
The second is the chemical indicator monitoring . Using chemical indicator in a certain temperature and reaction time under the conditions of heat discoloration or deformation characteristics , to determine whether the parameters required to achieve sterilization . Commonly used are:
Self- temperature tube: crystals of certain chemicals sealed in a glass tube ( length 2cm, diameter of 1 ~ 2mm) is made. Common agents are benzoic acid ( melting point 121-123 ℃) and so on. Sterilization , when the humidity rises to the melting point of the medicine , i.e. melting of the crystal tube and afterwards, although the cooled and then solidified , and its shape is still unmelted crystalline phase difference, this method can only indicate the temperature , does not indicate whether the duration of heat have been met , and therefore minimum standards.Mainly used for monitoring the packaging center .
3M pressure sterilization indicator tape : This tape printed with instructions oblique white line pattern , is a sterile affixed outside of the package to be sterilized in a special color tape . Paste surface dressing can be firmly closed package , metal box or glass article of 20 minutes at 121 ℃ , 130 ℃ for 4 minutes after the tape 100% color ( black stripe pattern that appears diagonal stripes ) . 3M tape both for goods packaging surface condition monitoring , but also for the monitoring of the packaging center , you can also replace the pins, clips or tape to use .
The third is a biological indicator monitoring . The use of heat-resistant non-pathogenic bacterial spores as indicator bacteria to determine the effect of heat sterilization . Bacillus stearothermophilus strains with the bacteria Bacillus strong resistance to heat , the thermal death time and the strongest resistance to pathogenic microorganisms similar botulinum spores . Biological indicators are spore suspension Bacillus bacteria plate and sheet, and mixed medium of instruction pipe . Testing should use standard test packets, each packet center position biological indicator 2 , on the sterilizer chamber 5 points , namely , the middle point of the center of each one , the lower the before, during and after a point . After sterilization , remove the biological indicator , bromocresol purple inoculated glucose peptone water medium , set 55-60 ℃ incubator for 48 hours to 7 days observations final result. If the color unchanged after culture , clear and transparent , indicating that spores have been killed . Reached the sterilization requirements . If turns yellow turbid , say not to kill spores , sterilization failed.

More details, Please view http://www.autoclavesale.com/ website

What is an Autoclave?

What is an Autoclave?

Autoclaves are used in sterilization. The advantage of using an autoclave is that it can reach temperatures higher than boiling water alone, so it can kill not only bacteria but also bacterial spores, which tend to be resistant. Autoclaves are used in laboratories to assure items such as glassware and surgical equipment are sterile. Have a question? Get an answer from a Medical Professional now!

Significance

Autoclaves are built around the principal that the boiling point of water increases when it is under pressure. At 15 pounds of pressure per square inch, the boiling point of water increases from 100 degrees Celsius to 121 degrees Celsius. At this temperature, all life forms are killed within 15 minutes.

Features

Autoclaves are filled with water and work by creating steam within an enclosed environment, which builds up pressure. The air within the autoclave is gradually replaced with steam, which can reach higher temperatures than the air. High-temperature steam can surround and infiltrate the items, even reaching within the crevices in stainless steel instruments. This process kills all bacteria, viruses and bacterial spores.

Benefits

Autoclaving allows materials to be sterilized within a relatively short time frame without the use of reagents. It also allows objects such as surgical and dental equipment to be reused. Therefore, autoclaving is an environmentally friendly option.

Considerations

To assure the autoclave has sterilized the objects within it, it is important to note the maximum temperature and how long it was held there. There are also indicators that can be purchased and placed on the items in the autoclaves, which will change color when they have been held at the appropriate temperature for a certain length of time.

Medical equipment needs to be sterilized to prevent the spread of infection before it can be used. An autoclave is a device that sterilizes medical equipment. Autoclaves are also used by tattoo and piercing artists to sterilize needles. The autoclave developed as an extension of the research done with pasteurization processes.

History of Sterilization

Between 460 and 377 B.C. Hippocrates cleaned surgical instruments by pouring boiling water over them.

Between 1729 and 1799 Italian biologist Lazzaro Spallanzani discovered that bacteria died after being heated in sealed glass flasks for 30 minutes.

On April 20, 1862 Louis Pasteur conducted the first series of tests in which liquids where heated in pressurized containers to sterilize them. This became known as pasteurization and is still used to preserve milk, juice and other foodstuffs.

A rudimentary autoclave was first created in 1879 by French microbiologist Charles Chamberlain. He worked with the famed Louis Pasteur on his research into sterilization and pasteurization.

In its most basic form the autoclave is a pressure cooker. Water is heated in a pressurized environment to create steam. Using pressure makes it possible to heat to higher temperatures with less energy. Autoclaves are usually made of steel and have various configurations for removing air prior to pressurization. Downward displacement autoclaves use gravity to remove air. Steam pulsing autoclaves use pulses of steam along with pressurizing and depressurizing to reach optimum pressure. Vacuum pump autoclaves suck air out for pressurization. Superatmospheric autoclaves are a combination of steam pulsing and vacuum pump techniques.

How Does an Autoclave Work?

An autoclave sterilizes items by heating them with steam to a very high temperature. Some common temperatures at which autoclaves operate are: 115 degrees C/10 p.s.i., 121 degrees C/15 p.s.i., and 132 degrees C/27 p.s.i. (p.s.i.=pounds per square inch). The temperature, pressure and time of operation depend on the degree of sterilization needed.

What Does an Autoclave Kill?

An autoclave using standard settings can kill most bacteria, spores, viruses and fungi. However, most prions are not killed by an autoclave using standard settings and some organisms can survive at temperatures above 120 degrees C. Most doctor’s offices, tattoo parlors, dentist offices and other places where instruments might come in contact with contaminants have a small autoclave on site for disinfection.

Hospitals use larger autoclaves that look similar to industrial dishwashers to sterilize many items at once.
Heat kills microorganisms by causing vital proteins to coagulate. The proteins stick together causing fatal damage to the microorganism. An autoclave cooks microorganisms in the same way a pressure cooker cooks food, but at a higher temperature. Autoclaves use steam instead of dry heat because steam can more effectively transmit heat to the microorganisms.