High-pressure Steam Sterilization Precautions
High-pressure steam sterilization method is a rapid and effective method of sterilization . High-pressure steam sterilizer , generate steam by heating , with increasing steam pressure , a rise in temperature , typically the pressure 103.4kPa ( rather old system 15 lb / inch 2 or 1.05kg/cm 2) , the inside temperature up to 121.3 ℃, keep 15 ~ 30min, can kill all microorganisms , including spores , including . This method is commonly used in the general saline , surgical instruments and dressings and other goods humidity and high temperature sterilization .
High-pressure steam sterilization precautions :
First, the sterile package is not too large ( less than 50cm × 30cm × 30cm), should not be too tight , have a gap between each parcel , so that the steam can easily penetrate into the parcel central convection . Before disinfection , open tanks or boxes vent steam circulation in favor . And quickly exhaust the steam is discharged to keep the dry goods . When sterilization is completed, close the vent tank or cartridge to maintain sterility of the goods .
Second , cloth items should be placed on metal objects , otherwise the steam condensed into water droplets freeze so damp cloth . Hinder steam into parcels central , seriously affecting the sterilization effect.
Third, check the inspection sterilization effect periodically. After autoclaving sterile packs , sterile container to one week period is appropriate. Monitoring the effect of high-pressure steam sterilization : There are following three methods .
The first is the process monitoring ,also known as procedures monitoring. Depending on the installation on the sterilizer gauges ( pressure gauges, thermometers , Chronograph ) , charts, indicating needle , alarms , etc., indicate sterilization equipment is working properly or not. This method can be quick to point out sterilizer malfunction, but can not determine whether the items reach the sterilized sterilization requirements . This method as a routine monitoring methods , sterilization should be carried out every time .
The second is the chemical indicator monitoring . Using chemical indicator in a certain temperature and reaction time under the conditions of heat discoloration or deformation characteristics , to determine whether the parameters required to achieve sterilization . Commonly used are:
Self- temperature tube: crystals of certain chemicals sealed in a glass tube ( length 2cm, diameter of 1 ~ 2mm) is made. Common agents are benzoic acid ( melting point 121-123 ℃) and so on. Sterilization , when the humidity rises to the melting point of the medicine , i.e. melting of the crystal tube and afterwards, although the cooled and then solidified , and its shape is still unmelted crystalline phase difference, this method can only indicate the temperature , does not indicate whether the duration of heat have been met , and therefore minimum standards.Mainly used for monitoring the packaging center .
3M pressure sterilization indicator tape : This tape printed with instructions oblique white line pattern , is a sterile affixed outside of the package to be sterilized in a special color tape . Paste surface dressing can be firmly closed package , metal box or glass article of 20 minutes at 121 ℃ , 130 ℃ for 4 minutes after the tape 100% color ( black stripe pattern that appears diagonal stripes ) . 3M tape both for goods packaging surface condition monitoring , but also for the monitoring of the packaging center , you can also replace the pins, clips or tape to use .
The third is a biological indicator monitoring . The use of heat-resistant non-pathogenic bacterial spores as indicator bacteria to determine the effect of heat sterilization . Bacillus stearothermophilus strains with the bacteria Bacillus strong resistance to heat , the thermal death time and the strongest resistance to pathogenic microorganisms similar botulinum spores . Biological indicators are spore suspension Bacillus bacteria plate and sheet, and mixed medium of instruction pipe . Testing should use standard test packets, each packet center position biological indicator 2 , on the sterilizer chamber 5 points , namely , the middle point of the center of each one , the lower the before, during and after a point . After sterilization , remove the biological indicator , bromocresol purple inoculated glucose peptone water medium , set 55-60 ℃ incubator for 48 hours to 7 days observations final result. If the color unchanged after culture , clear and transparent , indicating that spores have been killed . Reached the sterilization requirements . If turns yellow turbid , say not to kill spores , sterilization failed.
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