Monthly Archives: November 2013

How to Use The Autoclave Processing Laboratory Waste

How to Use The Autoclave Processing Laboratory Waste

During the experiment produces many waste, which if not properly handled will be contaminated lab to lab work affected, and even endanger the health and lives of laboratory personnel , so laboratory waste disposal very important , following a brief introduction about the laboratory autoclave waste treatment methods and precautions :

a) the main laboratory will have to be prepared for all the different sizes into the sterilized waste in a plastic bag or a stainless steel tube , in the temporary storage and sterilization of all the waste must be placed into a bag and destroy bacteria disk. Bilayer before sterilization autoclave bags well in a volume of waste sterilization bag must not exceed the volume of the bag 3/ 4 , sterilized bag with a rubber band strapped tie too rigid but not otherwise steam can not enter inside the plastic bag impact sterilization effect. Orange disposable waste container in the above-described double sterilization bag during storage and sterilization must be kept secure place , the stainless steel waste container must be firmly and securely placed in a tightly closed sterile tray , each bag sterilization must be labeled with instructions to evaluate the sterilization effect.
b) between the experimental operation of all waste generated in situ sterilization process should be timely .Feel free to view to see more details. If the sterilizer is being used or due to other reasons not immediately used, the waste must be placed in the sterilization tray , and placed in sterile bags strapped between the experimental operation , the bag during storage to ensure no damage .
c) When the autoclave sterilization to time, in an external laboratory biosafety open the pressure cooker , remove the items on waste collection point .
d) When starting the sterilization must be satisfied that proper pot of water sterilization , high temperature and time are set correctly.

Classification of sterilization Methods

Classification of sterilization Methods

Biological sterilization have chemical and physical sterilization sterilization difference, physical sterilization is divided into dry heat sterilization, heat sterilization, radiation sterilization and filtration sterilization, dry heat sterilization can be divided into dry heat sterilizer, sterilization tunnel systems, radiation sterilization can be divided into radiation sterilization, ultraviolet sterilization; chemical sterilization is divided into gas and liquid sterilization sterilization, ethylene oxide gas sterilization including off bacteria and ozone sterilization, sterilization and other liquid sterilization including formaldehyde, as follows:

Sterilization methods:

1 physical sterilization of bacteria:

1) dry heat sterilization 2) moist heat sterilization 3) ray sterilization 4) filtration sterilization

2 chemical sterilization:

A) gas sterilization:

a b ozone sterilization ethylene oxide sterilization

2) liquid sterilization

More details,please view site.

Sterilization Process

Sterilization Process

About sterilization process :

Smart steam sterilizer operation is: heating ( cooling air emissions during this process ) – sterilization (steam saturation detection system after the saturated steam into the interior of the process , the solenoid valve in the process is always off state , the smart sterilization process temperature, high pressure steam is not discharged , the inner loop soda ) – exhaust (exhaust cooling after sterilization is different depending on the mode of the process ; liquid sterilizing mode to take the exhaust gas pulsation , natural cooling mode, the exhaust gas is not taken natural cooling manner ; sterilization if solid , the user can press on the keypad keys to force open the valve , the exhaust gas to accelerate the rapid cooling rate ) .

Sterilization process has an advanced technology , with saturation vapor detection system to ensure that the inner container reaches pure saturated steam sterilization process temperature control in combination , to avoid a similar product without steam saturation detection system and the use of pressure control which led display temperature incompatible with the internal real ( internal temperature is higher than the true temperature display ) reach the sterilizing effect of defects ; 2, the sterilization process using smart thermostats , not through the discharge temperature steam thermostat . Advantages smart thermostat that : a temperature accuracy, volatility is small. b the soda cycle , saving water and electricity consumption, reduce energy waste.

About Sterilization mode:

There are three modes of sterilization : a pulsating cooling mode , after the pulsating exhaust gas sterilization, suitable sterile liquid substance; 2, the natural cooling mode , not vented after sterilization , natural cooling ; 3 , warm mode after the end of the sterilization can be performed according to user settings incubated between 45-60 ℃.Check more details on .

About sterilization procedures

Each mode has three programs , namely: sterilization temperature 135 ℃, sterilization time 5min; sterilization temperature 121 ℃, sterilization time 30min; sterilization temperature 115 ℃, sterilization time 40min, these three programs can edit the user’s needs , and can be automatically stored.

Sterilizer Selection

Sterilizer Selection

First, high pressure steam sterilizer working principle

High pressure steam sterilization is the article to be sterilized in a sealed pot pressure sterilization by heating , sterilization pot of boiling water and steam is generated . Water vapor will be sharp cold air from the pot in the drive to make the exhaust valve , then close the exhaust valve , heating was continued at this time because the steam can not overflow , and increasing the pressure within the sterilizer , thereby increasing the boiling point , obtained with the high temperature of 100 ℃ . Cause bacterial protein denaturation and coagulation to achieve the purpose of sterilization.

Second, the advantages of high-pressure steam sterilization

Autoclave sterilization methods are now widely used , and it has quick and efficient , to achieve convenience , low cost, no side effects and other advantages.

At the same temperature , heat than dry heat sterilization efficacy big . There are three reasons : First, the bacterial cell absorbs heat moisture, protein coagulation easier , because the water content increased protein required solidification temperature decreases , the second is the penetration of heat than dry heat large , three steam heat there is the presence of latent heat . 1g of water at 100 ℃, can emit 2.26KJ ( kJ ) when the heat from the gas into a liquid . This latent heat , can rapidly raise the temperature of the object to be sterilized , thereby increasing the effectiveness of the sterilization .

When using high pressure steam sterilization , are excluded completely cold sterilization pot is extremely important because the air pressure is greater than the expansion of the expansion pressure of water vapor , so that when water vapor contained in the air at the same pressure , comprising air temperature below the saturation temperature of the steam vapor .Wanna know more?Please visit .

Third, errors the average user understand the sterilizer

A considerable part of the user is considered sterilizer cooker , as long as the pressure reaches the sterilization effect can be achieved , this view is wrong , as the air compressor can produce a very high pressure , but not the function of sterilization . Sterilization is not a major factor in the pressure but the temperature of the steam saturation temperature plays a decisive role, and depends on the degree of saturation vapor emissions are completely cold .

Therefore, when using high-pressure steam sterilization , are excluded completely cold sterilization pot is extremely important because the air pressure is greater than the expansion of the expansion pressure of water vapor , so that when water vapor contained in the air at the same pressure , the steam temperature is lower than air containing saturated steam temperature.

Common Sterilizer Control Mode

Common Sterilizer Control Mode

Microcomputer intelligent automatic control, control sterilization pressure, temperature and time;See more details on .
Automatic over-temperature protection device: more than the set temperature, the heating is automatically cut off the power supply;

Door safety interlock device: cavity pressure, door can not be opened, patented device;

Low water level alarm: When water can automatically cut off the power, sound and light alarm, import without water detection device;

Leakage protection: Configuration leakage protection device;

Dynamic digital display temperature sterilization end end signal emitted;

Heating, sterilization, exhaust, automatic control of the drying process, without human supervision;

Mode 1: heating – sterilization – exhaust;

Mode 2: heating – sterilization – Pulse exhaust;

Mode 3: heating – sterilization – no exhaust,

Mode 4: Custom.

P3 Laboratory Sterilizers Introduction

P3 Laboratory Sterilizers Introduction

P3 laboratory biosafety level three laboratory. Biological safety laboratory according to the damage degree of different microorganisms and their toxins, can be divided into 4 levels, one level is the lowest, the highest level four. A laboratory is generally applicable to non pathogenic role in healthy adult microorganism; two level suitable for medium potentially harmful microorganisms on people and the environment; three level applies mainly through respiratory pathways that people infected with severe or fatal disease pathogenic microorganisms or their toxins; four level suitable for dangerous height on the human body, spread by aerosol path or route of transmission is unknown, the pathogenic microorganism is no effective vaccine or treatment or its toxin.

The P3 laboratory is the highest laboratory safety protection, requirements for laboratory is also very high, usually require autoclaves, inoculating loop sterilizer, epoxy ethane sterilizer, hydrogen peroxide plasma sterilization etc.. Due to the sterilization products requiring high and not every brand can satisfy this requirement, if the construction of the laboratory, especially the international certification P3 laboratory is best to buy imported products, of course, such costs are very expensive, below I will see about for P3 lab products.


The first is the German Systec products, as the world’s best sterilizer company, Systec is the first to bear the brunt of the double door series, DS is an excellent choice for P3 laboratory, double door in full compliance with the P3 laboratory sterile requirements, but know the price before you are fully prepared oh, 90L sterilizer dealers purchase price 30000 euro, 150L to 40000 euros to 50000 euros, 200L.Feel free to click on to get details. Germany MBT autoclaves and the United States of America, Terry set can also select the range, the price is slightly lower, but converted into yuan to several hundred thousand dollars.

Dry heat sterilizer — inoculating loop sterilizer

The United States of America KENDALL inoculating loop sterilizer is designed for the P3 laboratory, with professional certification, the price is very high, dealers to buy to 5000 yuan, the market price is 5500, while the ordinary import dry heat sterilizer price as long as 2000 yuan, almost no profit, but regular P3 laboratory is the best choice of the sterilizer.

Ethylene oxide sterilizer

It can be applied to the P3 Laboratory of ethylene oxide sterilizer, only Japan and the United States of America Misaki Sato 3M production, the price is very high, with ethylene oxide sterilizer price to $500000 Misaki Sato 250L double door P3 laboratory, while 3M to more than million, because the price is too high, so the two manufacturers produced little door products, but can according to the customer request custom-made

The P3 laboratory is a stranger that not well-known to the world, a fan of thick door let this little lab isolated from the outside world, mysterious and quiet, the products also have very high demand, product price high is worth, if you are preparing to construct the P3 laboratory to choose sterilization equipment, please find us, we provide the cheapest product safety performance of P3 laboratory highest for you, pack your satisfaction! Days were medical provide first-class products for your lab!

Comparison of Several Sterilizers

Comparison of Several Sterilizers

Many different types of sterilization , heat sterilization is the most commonly used method, but ethylene oxide sterilizers and ozone sterilizer in industrial and medical applications is also a great use , now the following types of sterilization of these products at simple comparison , for you to choose the right type of sterilization :

Dry Heat Sterilizer

High pressure Autoclave

Ethylene oxide sterilizers

Ozone Sterilizer


Heating the article in dry air , to kill microorganisms

Goods by the pressure inside the sterilization steam or other heat sterilization medium to kill microorganisms .More details,please view .

The use of ethylene oxide gas for sterilization strong oxidation method

The use of ozone at room temperature under atmospheric pressure rapidly self-decomposition into oxygen (O2) and a single oxygen atom (O), which has a strong activity , bacteria have a strong oxidation , and to achieve a sterilization effect methods .


Ordinary biological laboratory sterilization , withstand high temperatures , can not easily be susceptible to heat or steam penetration items are broken ring

Widely used, most commonly used in biology , all high pressure and heat when the temperature does not change this method or damaged items can be sterilized

Industrial sterilization, heat intolerance medicine, medical equipment , facilities , and equipment sterilization

Industrial sterilization, heat intolerance medicine, medical equipment , facilities , and equipment sterilization

Common areas

Common laboratory , food processing plants , hospitals conventional instrument sterilization , such as pharmaceuticals, liquid, glassware , dressings, fabric , metal equipment and other products

Sterilized clothing industry , pharmaceutical industry, drug intolerance heat sterilization , hospital disposables sterilization , biological engineering, food processing, chemical sterilization of packaging materials

Product Use

Simple, according to their own operations manual

Complex and require specially trained personnel in under the guidance of

Price Range

Made in China

Mostly in the 10,000 yuan or less, about a few products price 20000

Less than 5,000 yuan

100 thousand

Larger sales are around 40,000 yuan

20 thousand

More than 100 thousand

Relevant parameters

Temperature, humidity, sterilization time

Temperature, pressure , humidity, sterilization time

Temperature, humidity, gas concentration , sterilization time

Humidity, temperature, gas concentration , sterilization time

Internet sales practicable degree

Suitable for online sales

Suitable for online sales

Suitable for online sales

Suitable for online sales


Physical sterilization

Physical sterilization

Chemical sterilization

Chemical sterilization

Desired temperature sterilization

High (160 ℃ above )

In (100 ℃ -130 ℃)

Low (under 60 ℃ )

Low (under 60 ℃ )

The time required for sterilization ( sterilization items into the sterilizer from the inside to remove the time )

In ( within two hours )

Short ( tens of minutes )

Long ( three hours or more )

Long ( three hours or more )






Portable, Vertical, Horizontal Autoclaves Distinction

Portable, Vertical, Horizontal Autoclaves Distinction

Sterilizer generally can be divided into portable autoclaves, vertical and horizontal autoclaves , which in the end is how to distinguish between, for what is now described as following comparison,you can also figure out by following .

1, the portable autoclave :

Capacity : 20L or less, more for the 18L and 12L

Degree of automation : Low ( mostly semi-automatic )

Appearance: cylinder

Opening direction : Upward

Energy requirements: coal or electricity

Temperature: up to 126 ℃ generally

2 , vertical autoclave

Capacity : 30L-100L, lots of model range

Degree of automation : high ( according to user need to select the different models )

Appearance: Cube

Opening direction : Upward

Energy requirements: Electric

Temperature : up to 134 ℃ generally

3 , horizontal autoclave

Volume : 100L above

Automation : High ( mostly automatic )

Appearance: cylinder

Opening direction : forward

Energy requirements: Electric

Temperature : up to 134 ℃ generally

These three autoclaves main difference is the size and function , the portable sterilizer relatively simple to poor performance , small size , low price ; while in addition the difference between vertical and horizontal sizes, and shapes and the opening direction , personally think that the volume of 30L-100L Sterilizer mostly in height 700mm or so, so open the door upward, convenient operation, designed as a cube more beautiful and can be placed easy. The horizontal sterilizer is larger , if the design formula height was established too high, opening inconvenient. As the same size of the largest surface area of ​​the sphere , so horizontal sterilizers volume is generally larger than 100L .

Brief Introduction of Pulse Vacuum Steam Sterilizer Working Theory

Brief Introduction of Pulse Vacuum Steam Sterilizer Working Theory

Pulse vacuum steam sterilizer referred to as pre-vacuum pressure cooker. Is the use of a vacuum pump to the pot becomes exhausted air vacuum state, and then import the steam, the steam in the pot quickly spread and penetrate deep into the pot and goods to achieve effective sterilization.Check more details on .

Compare with the high pressure steam boiler has significant advantages:

⑴ sterilization time is about 1/3 of high-pressure steam pot’s;

⑵ sterilization effect is more reliable than high-pressure steam, and easy to operate;

⑶ less damage of sterilizing goods;

⑷ sterilized dressing kits are close to dry, can be used immediately after taken out.

Autoclave Air Exclusion

Autoclave Air Exclusion

Inside the autoclave steam temperature not only relate with the pressure , but also on the degree of saturation and steam . If the pressure cooker or complete exclusion of air is not excluded , the steam can not reach saturation , although the expression of the sterilized pounds pressure, but the steam temperature has not reached the required one, resulting in the failure of sterilization .

Autoclave sterilization air can also affect the sterile steam to penetrate . Saturated steam can penetrate to the center of dressings , when the air is not completely excluded, the low temperature to form the package , the package outside temperature is high , so that can not be achieved within the sterilization.

Check whether the air sterilizer drain is: at the exit of the exhaust pipe connected to a hose , the other end into a pot of cold water , if the tube exhaust gas bubbles in cold water , then drain yet .Feel free to visit .Need to continue to exhaust ; Without bubbles, it means that the pot of cold air has been basically drained . If the device to be sterilized have a certain amount of steam and then exhaust the air purged in favor .

In the exhaust process, sometimes the exhaust duct may be blocked by foreign body , then you can observe the exhaust pipe thermometer indicates the temperature and pressure values ​​calculated by the pound table the temperature is close enough . Temperature by the simple pressure pounds conversion formula is:

Temperature (℃) = pounds pressure gauge indicates +106

For example: pound table indicates a pressure of 15 pounds in the case of pure steam , the pot temperature should be 15 +106 = 121 ℃. If the thermometer indicates that the temperature is lower than 121 ℃, then the pot is not purged of air , the exhaust duct may be a foreign body obstruction , should be excluded.