Monthly Archives: November 2013

Autoclave Maintenance And Use

Autoclave Maintenance And Use

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1 , routine maintenance
1.1 sterilizer appearance and sterilizing chamber to keep clean and dry.
1.2 probes, water level should be cleaned regularly .
1.3 doorframe , apron without damage , steam inlet must not be blocked . Preferably every day after use in strips coated talc. To extend the tape life.
1.4 The interlock device to be flexible , reliable, open freely .
1.5 traps cleaned once a month , in order to facilitate discharge air to maintain the temperature .
2, the maintenance cycle
After one year , every year to ask a qualified inspection department to do a comprehensive and systematic examination . Including : Simplified , doors , piping systems , electrical systems. Safety valves, thermometers , pressure gauges should regularly check to ensure equipment safety and proper use .
3 , parking
Such as long-term parking , the device should be placed in ventilated, dry place, not the rain , when necessary, a shelter . Be drained water in the steam generator , and the door is open , sterilizing chamber to be kept clean and dry .Check more details on .
4 Precautions for daily use
4.1 sterilizer should be trained and qualified personnel to operate , the entire sterilization process should be supervised .
4.2 can not fully rely on automatic water level protection , you should always pay attention to the water level , so as not to burn out the heating pipes .
4.3 When artificial water . Should cut off the power , open the pressure relief vent valve 3 , again entered the water valve . Do not open when there is pressure on the mezzanine inlet valve , add water when the vent valve should be left open .
4.4 When the sterilization chamber pressure , the interlock lever can not be raised, can not be forced to open the door .
4.5 pairs of liquid sterilization should slow release steam . Until the liquid temperature drops below 700C in order to open the door. Prohibit door immediately after sterilization .
4.6 sterilization process if there is a power failure or other causes than sterilization temperature, sterilization temperature should be reached again when the temperature begins timing .
4.7 When there is pressure on the mezzanine weekly pull valve handle several times to ensure that the safety valve is working properly.
4.8 Daily classes will sterilizer glove plate away, with a dry sterile cloth set and maintain sterilization chamber dry.
4.9 Class day after the steam generator water with pressure discharge end, in order to reduce the scale .
4.10 Chang Lu equipment operation should not be required to build when Chang Lu protective frame , to prevent rain .
4.11 Continuous use of time should be less than 8 hours / day .

How To Calculate Sterilization Time Properly

How To Calculate Sterilization Time Properly

High-pressure steam sterilization time calculation , should meet the requirements of sterilizer cabinet interior temperature , counting to sterilization is completed. Total time includes : thermal penetration time, ie autoclave sterilization temperature to reach the center of the sterile sterilization temperature has reached the desired time .Check it out on .Time depends on the nature of sterile packaging size , placement and autoclave sterilizer air emptying the extent and kind . Sterilization duration , i.e. the time required to kill microorganisms , usually with Bacillus stearothermophilus spores kill time required to represent.

When at 115 ℃ 30 minutes , 121 ℃ is required 12 minutes , 132 ℃ is required 2 minutes . Safety time , usually half the time to maintain , depending on its length may be sterile . Metal equipment for heat easily sterilized , no safe time .

Usually the exhaust time required for autoclaves : 115 ℃ 30minutes , 121 ℃ 15minutes , 126 ℃ 10 minutes. 115 ℃ commonly used in the pharmaceutical industry sterilization . In the microbiological laboratories , some sugar medium are sterilized with 115 ℃ 30minutes , because at higher temperatures decompose sugar . 121 ℃ ~ 126 ℃ commonly used in the health and epidemic prevention work in the sterilization. Knowledge of the sterilizing time of the above approximate number , 1 sterile specifically how much time , the need to the types of items , size of the package , put the situation and to determine the performance of sterilization .

How To Monitor The Effect Of High Pressure Steam Autoclave

How To Monitor The Effect Of High Pressure Steam Autoclave

How to monitor the effect of high pressure steam autoclave?Need a certain monitor standard,got three following ways:

First is the technology monitoring,called program monitoring.Based on the autoclave installed gauge(pressure gage,thermometer,timer),chart,indicator,alarm ect,to check autoclave working or not.This method can point out the bug of autoclave promptly,but not make sure if the goods to be sterilized reach the sterilizing requirement.This method as a routine monitoring method, should be carried out every time sterilization.

The second is the chemical indicator monitoring . Using chemical indicator in a certain temperature and reaction time under the conditions of heat discoloration or deformation characteristics , to determine whether the parameters required to achieve sterilization .

Commonly used are : 1 ) self- temperature tube: crystals of certain chemicals sealed in a glass tube ( length 2cm, diameter of 1 ~ 2mm) is made. Common agents are benzoic acid ( melting point 121-123 ℃) and so on. Sterilization , when the humidity rises to the melting point of the drug , i.e. melting of the crystal tube and afterwards, although the cooled and then solidified , and its shape is still unmelted crystalline phase difference, this method can only indicate the temperature , does not indicate whether the duration of heat have been met , and therefore minimum standards. Article is mainly used for packaging the monitoring center .

2) 3M pressure sterilization indicator tape : This tape printed with instructions oblique white line pattern , is a sterile affixed outside of the package to be sterilized in a special color tape . Paste surface dressing can be firmly closed package , metal box or glass article of 20 minutes at 121 ℃ , 130 ℃ for 4 minutes after the tape 100% color ( black stripe pattern that appears diagonal stripes ) . Click to check more .3M tape both for goods packaging surface condition monitoring , but also for the monitoring of the packaging center , you can also replace the pins, clips or tape to use .

The third is a biological indicator monitoring . The use of heat-resistant non-pathogenic bacterial spores as indicator bacteria to determine the effect of heat sterilization . Bacillus stearothermophilus strains with the bacteria Bacillus strong resistance to heat , the thermal death time and the strongest resistance to pathogenic microorganisms similar botulinum spores . Biological indicators are spore suspension Bacillus bacteria plate and sheet, and mixed medium of instruction pipe . Testing should use standard test packets, each packet center position biological indicator 2 , on the sterilizer chamber 5 points , namely , the middle point of the center of each one , the lower the before, during and after a point . After sterilization , remove the biological indicator , bromocresol purple inoculated glucose peptone water medium , set 55-60 ℃ incubator for 48 hours to 7 days observations final result. If the color unchanged after culture , clear and transparent , indicating that spores have been killed . Reached the sterilization requirements . If turns yellow turbid , meaning spores not killed , sterilization failed.

What Is High Pressure Autoclave And Its Uses?

What Is High Pressure Autoclave And Its Uses?

What is the autoclave and uses? Autoclave also known as high-pressure steam sterilization pot, can be divided into portable autoclaves and vertical autoclaves. More details,please view site.

The device Using electric wire to heat water to produce steam, and to maintain a certain pressure. There are a barrel can be sealed, pressure gauges, exhaust valve, safety valve, electric wire and other components. For medical and health services, scientific research, agriculture and other units, medical equipment, dressing, glassware, media and other solutions for disinfection and sterilization, is the ideal device.

Portable Autoclave is a necessary testing equipment for food factory, drinking water plants do QS, HACCP certification .

How to Use the Sterilizer

How to Use the Sterilizer

1 Add the right amount of water into the outer pot, put items need to sterilized into the inner pot, cover the lid and tighten the screw symmetrically.

2 Heat pot to produce steam , when the gauge pointer reaches 33.78kPa, open the exhaust valve, release the cold air ,then the pressure gauge pointer goes down, when it goes down to 0, close the exhaust valve.Check more on

3 Continue heating, steam in pot increases, pressure gauge pointer goes up again when the pot pressure hit the desired pressure, reduce the fire, according to the characteristics of the sterilized items, keep the vapor pressure a certain time as required, and then power off or fire off the sterilizer, let it cool and then slowly open the exhaust valve to exclude residual air before opening the lid to extract.

Vertical Pressure Steam Sterilizer Features

Vertical Pressure Steam Sterilizer Features

Vertical Pressure Steam Sterilizer Material:

Outer and inner cavity , door , sterilization baskets are made of high quality stainless steel SUS304 , acid, alkali, corrosion ;

Vertical Pressure Steam Sterilizer Product Configuration :

Safety valve: over the rated pressure , release the pressure ;

Hand wheel : Rotating compass -style open door , simple and convenient ;

Gauge : Indicates pressure display ;

Seal : Self- inflating type seal ;

The bottom of the wheels ;

Medical Device Registration Certificate with product , each through rigorous testing and Technical Supervision ( each device with a check

Test certificate )

Vertical Pressure Steam Sterilizer control:

Microcomputer intelligent automatic control, control sterilization pressure , temperature and time ;

Automatic over-temperature protection device: exceeds the set temperature , automatically cut off the heating power ;

Door safety interlock : cavity pressure, door can not be opened , patented device ;

Low water level alarm : when water can automatically cut off the power , sound and light alarm , imported without water detection device ;

Leakage protection : Configuring leakage protection device ;

Dynamic digital display temperature , sterilization issue completion signal the end ;
Check more details on
Vertical Pressure Steam Sterilizer Technical parameters:

Control mode: external circulation next row ;

Microcomputer automatic control of heating sterilization procedures LED digital display ;

Volumetric : 100L;

Timing range :0-999min;

Power supply voltage : 220V/50Hz;

Heater power : 3.5KW;

Sterilization chamber size : (D = 360 H = 240) * 3 month;

Dimensions: 500 * 500 * 1300 ;

Maximum design pressure / temperature : 0.25MPa/138 ℃;

Maximum operating pressure / temperature : 0.22MPa/136 ℃;

Dimensions : length * D * H ( dimensions mm: mm ) D: that diameter H: indicates altitude ;

Operating temperature : ± 5-35 ℃.

Using Autoclave Handle Laboratory Waste

Using Autoclave Handle Laboratory Waste

Experimental process will produce many wastes, if not properly deal with these wastes will contaminate lab to get lab work affected, or even be harmful to the health and lives of laboratory personnel , so laboratory waste disposal is very important , the following are brief autoclave laboratory waste treatment methods and precautions :

a) In the main laboratory will have to be ready all the waste into sterile plastic bags of different sizes or stainless steel tube , in the temporary storage and sterilization , all waste must be placed in the bag and placed in an off bacteria tray. Before sterilization autoclave bags double wrap, the volume of the waste bag in a sterilization bag, the volume should not exceed 3/ 4 , sterilized bag with a rubber band strapped , but not too rigid bar or steam not affect the sterilization effect into a plastic bag .Check details on .Orange disposable waste container on the double sterile bag during storage and must be kept safe place during sterilization , stainless steel waste containers must be tightly closed firmly and securely into sterile tray, each bag must be labeled with instructions to evaluate the sterilization sterilization effect .
b) The experimental operation of all waste generated in sterilization process should be timely . If the sterilizer is being used or for other reasons can not be used immediately , the waste must be placed within the sterilization tray , sterilization bag strapped experimental operating room and placed within the bag during storage to ensure no damage .
c) When the autoclave sterilization is accomplished, open the laboratory biosafety pressure cooker outside, remove items on waste collection point .
d) When starting the sterilization must make sure that proper sterilization pot of water , high temperature and time should be set correctly.

How To Use Autoclaves In A Safe Way

How To Use Autoclaves In A Safe Way

A. Items to be sterilized should not be placed too tight;

B. Cold air must be fully ruled out, otherwise the pot temperature is less than the required temperature, affecting the sterilization effect;

C. After sterilization, do not deflate decompression, or bottle of liquid will vigorously boiling, flush spill stopper and even cause the container to burst. Click to know more.Subject to the sterilizer is equal to atmospheric pressure dropped only after opening the lid;

D. Now has microcomputer automatic control of high-pressure steam sterilizer, simply release air, the instrument automatically maintain constant time, when the time comes automatically cut off the power and whistle, very easy to use.

Moist Heat Sterilization And Dry Heat Sterilization Difference

Moist Heat Sterilization And Dry Heat Sterilization Difference

( One ) dry heat sterilization

1 . Incineration : a thorough sterilization methods , but only for goods or abandoned corpses .

2 . burning : direct flame sterilization, for microbiology laboratory inoculation loop , tube mouth, such as sterilization .

3 dry roasted : oven sterilization. Generally heated to 160 ℃ ~ 170 ℃ for 2 hours . For high temperatures without deterioration , do not evaporate items,

Such as glassware, porcelain and so on.

( Two ) moist heat sterilization

1 Boiling : 1 atm , the boiling temperature of 100 ℃, generally of bacteria is killed boiled for 5 minutes . Check more details on .Bacterial spores often need to boil 1 to 2 hours before she was killed. Water by adding 2% sodium carbonate , can improve the boiling point of 105 ℃, promoting bacterial spores kill , but will also prevent rusting metal containers .

2 pasteurization (pasteurization): a liquid with a lower temperature to kill pathogens without compromising sterile nutrients and flavor disinfection methods . Heating 61.1 ~ 62.8 ℃ half an hour , commonly used in the disinfection of milk and wine, etc. .
3 intermittent sterilization method (fractional sterilization): is the use of repeated circulation of steam heating, kills all vegetative bacteria and spores sterilization . Specifically, the items to be sterilized is placed Arnold circulation steam sterilizer , 100 ℃ heating for 15 to 30 minutes, which kills bacteria, and then items placed in 37 ℃ incubator overnight to develop spores propagules into the next day and then through the circulation of steam heating, so three times in succession , you can have all bacteria and spores kill all .

4 high-pressure steam sterilization : sterilization effect is the best , the most widely used method of sterilization . Method is a closed steamer – Autoclaves conducted within . Heating the steam can not leak out, with the temperature in the reactor increases with the vapor pressure , sterilization is greatly enhanced. Usually 1.05kg/cm2 pressure, temperature of 121.3 ℃, for 15 to 30 minutes, including bacteria to kill all microorganisms , including spores .

This method is suitable for high temperature and humidity are not afraid of sterilizing.

Procedure of Using High Pressure Steam Sterilizer

Procedure of Using High Pressure Steam Sterilizer

  1. First, take out the inner basket, then add right amount of water to the outer pot, so the water level is appropriate phase with the triangular shelves.
  2. Back into sterilized barrels and loaded items to be sterilized. Be careful not to install too crowded, so as not to impede steam circulation and affect sterilization effect. Erlenmeyer flasks and test tube ends are not in contact with the tank wall to avoid condensation and wet paper bag mouth penetration tampon.
  3. Stamped, and insert the cover of the exhaust hose into the inner barrel sterilization exhaust slot. More details,please view .Then twenty-two relatively symmetrical manner while tightening the two bolts, the bolt tightness consistent, must not allow leakage.
  4. With electric or gas heating, and also open the exhaust valve, so that the pot of boiling water to eliminate the cold air. Subject to the cold air is exhausted, close the exhaust valve, so that the temperature of the pot with the steam pressure increases gradually increased. When the pressure is raised to the desired pressure pot, control heat, maintaining pressure to the desired time. In this experiment takes 1.05kg/cm2, 121.3 ℃, 20 minutes sterilization.
  5. After sterilization time required to cut off the power supply or turn off the gas, so that the natural decline in the pot temperature sterilization, when the gauge pressure dropped 0:00 to open the exhaust valve, unscrew the bolt and open the lid, remove the sterilized items. If the pressure is not reduced to 0 when the exhaust valve opens, a sudden pressure drop will be due to the pot, the medium within the container and the external pressure imbalance out port or tube mouth flask, resulting in the occurrence of the tampon contaminated medium contamination.
  6. The medium was removed into sterile 37 ℃ incubator for 24 hours, after inspection without bacteria growth, you can set aside.