First, the heating method :
Dry heat method : flame burning hot air sterilization and electric steam steriliser.
A flame burning applies to ring vaccination , inoculation needle and metal utensils such as tweezers , tube and bottle mouth , coated with a glass rod .
2 , hot air sterilization using high-temperature dry air (160-170 ℃) heat sterilized 1-2h, suitable for glassware and dish and so on. Principle heating denatures proteins , and water content , and when the larger environment and cell water content , the faster solidification .
3 , note:
1 ) medium , rubber products, plastic products can not use this method ;
2 ) temperature controlled at <180 ℃;
3 ) Items not too crowded ;
4 ) when the temperature was lowered to 70 ℃ box door.
Second, heat method :
Principle: Because the bacterial heat -absorbent, easy coagulation proteins , due to increased water content of proteins required solidification temperature decreases ; humid steam latent heat exists. So the effect of dry heat better than the same temperature .Stay tuned on http://www.autoclavesale.com/ .
Third, the high-pressure steam sterilization method :
1 Equipment: Autoclave
2 , the length of time depending on the type and number of different variations of sterilized items .
3 , suitable for sterilization medium , overalls , rubber goods, etc. , can also be used for sterilization of glassware .
4 Note :
1 ) cold completely rule ; 2 ) water ; before opening 3 ) pressure drop to “0” .
Fourth, the atmospheric steam sterilization :
Should not be used for the medium pressure steam sterilized medium such as gelatin , milk medium , this method can be sugar culture medium . Thorough sterilization is intermittent sterilization methods.
Boiling sterilization method :
Applicable syringes and dissected vessels, time 10-15min,
135-150 ° C and 2-8s for milk and other liquid food .
Advantages : Keep food value and nutrients.
Fifth, the filter sterilization :
Principle: When the medium pressure barrier microorganisms.
Scope: Many materials such as serum , antibiotics and sugar solution using this method .
Equipment : Chua filters, membrane filters.
Advantages and disadvantages: not destroy medium components for only a small filtrate. Require a lot of sterile air and clean bench .
Note: 1 , the pressure appropriate ;
2 , under sterile conditions ;
3, to prevent infiltration phenomenon .
Sixth, radiation sterilization :
Principle: UV wavelength at 200-300nm, has a bactericidal effect , with strong bactericidal 265-266 . Nucleic acid susceptible cells paragraph absorption wavelength ; can produce ozone and hydrogen peroxide . Bactericidal effect and is proportional to the product of intensity and time .
Cons: little penetration . From irradiated <1.2m appropriate. Applications: sterile room , hospital.
Note: eye and skin irritation.
AUTOCLAVESALE steam autoclave sterilizer: r -rays , penetrating power , suitable for sterilization stacked items.
Seven chemical sterilization :
Principle : The application of chemical agents destroy bacteria metabolism .
Fungicides such as heavy metal ions.
Antibacterial agents such as sulfa and most antibiotics.
Note : the level of concentration of drugs , length of time , in which microbial species and microbial environment.
Common chemical fungicides are: ethanol , acetic acid, carbolic acid
Formalin , mercuric chloride , potassium permanganate, Bromogeramine