How to Calibrate an Autoclave shared by Autoclavesale.com

Medical equipment is commonly sterilized in autoclaves. They are also used in microbiology laboratories. Autoclaves are available in many sizes. The smallest is a stovetop pressure cooker. Like our model PA-BM Portable Autoclave-Bluestone Autoclave. Countertop models are used in dentist’s offices and small medical clinics. Like our model TS-DDZ Dental Autoclave-Bluestone Autoclave. Large solid-state controlled autoclaves are common in laboratories and hospitals. Like Model HA-BC Horizontal Medical Autoclave-Bluestone Autoclave. All autoclaves have temperature and pressure gauges, as well as a timer. Quarterly calibration of the autoclave timer and gauges is important to insure that proper sterilization is occurring.

Portable Autoclave PA-BM_ BLuestone Autoclave

Portable Autoclave PA-BM_ BLuestone Autoclave

Dental Autoclave TS-DDZ - Bluestone Autoclave

Dental Autoclave TS-DDZ – Bluestone Autoclave

Horizontal Autoclave - Bluestone Autoclave

Horizontal Autoclave – Bluestone Autoclave

Timer Calibration

  1. Set the timer on the autoclave for a full cycle. Hold the stopwatch and be prepared to click it.
  2. Turn on the autoclave and the stopwatch simultaneously.
  3. Repeat the process if the time and the stopwatch do not match.
  4. If the autoclave timer differs from the stopwatch time by more than 30 seconds, post an appropriate correction factor directly on the autoclave.
  5. Note the calibration results in a logbook.

Temperature Calibration

  1. Place the maximum registering thermometer near the built-in temperature sensor.
  2. Load and operate the autoclave as usual.
  3. Record the maximum autoclave temperature according to the built-in temperature display.
  4. Let the autoclave cool.
  5. Remove the maximum registering thermometer from the autoclave and record the temperature reading in the logbook.
  6. Record the discrepancy if the maximum temperature of the built-in display is different from the maximum temperature on the registering thermometer.
  7. Post a correction factor on the autoclave control panel if the temperatures were different.

Pressure Calibration

  1. Calibrate the timer and temperature gauges before these steps.
  2. Load and operate the autoclave as usual. When the temperature display shows 121 degrees Celsius, the pressure gauge should show 15 pounds per square inch (15 psi).
  3. Record the discrepancy if there is any.
  4. Mark the correct point for 15 psi on the cover of the gauge and post a correction factor on the autoclave if this is not true.

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