How to Calibrate an Autoclave?

Medical equipment is commonly sterilized in autoclaves. They are also used in microbiology laboratories. Autoclaves are available in many sizes. The smallest is a stovetop pressure cooker. Countertop models are used in dentist’s offices and small medical clinics. Large solid-state controlled autoclaves are common in laboratories and hospitals. All autoclaves have temperature and pressure gauges, as well as a timer. Quarterly calibration of the autoclave timer and gauges is important to insure that proper sterilization is occurring.

Vertical Autoclave

Vertical Autoclave

Timer Calibration

  • Set the timer on the autoclave for a full cycle. Hold the stopwatch and be prepared to click it.
  • Turn on the autoclave and the stopwatch simultaneously.
  • Repeat the process if the time and the stopwatch do not match.
  • If the autoclave timer differs from the stopwatch time by more than 30 seconds, post an appropriate correction factor directly on the autoclave.
  • Note the calibration results in a logbook.
  • Temperature Calibration
  • Place the maximum registering thermometer near the built-in temperature sensor.
  • Load and operate the autoclave as usual.
  • Record the maximum autoclave temperature according to the built-in temperature display.
  • Let the autoclave cool.
  • Remove the maximum registering thermometer from the autoclave and record the temperature reading in the logbook.
  • Record the discrepancy if the maximum temperature of the built-in display is different from the maximum temperature on the registering thermometer.
  • Post a correction factor on the autoclave control panel if the temperatures were different.
  • Pressure Calibration
  • Calibrate the timer and temperature gauges before these steps.
  •  Load and operate the autoclave as usual. When the temperature display shows 121 degrees Celsius, the pressure gauge should show 15 pounds per square inch (15 psi).
  •  Record the discrepancy if there is any.
  •  Mark the correct point for 15 psi on the cover of the gauge and post a correction factor on the autoclave if this is not true.
    Vertical Autoclave

    Vertical Autoclave

    More information about autoclave,please view the website: http://www.autoclavesale.com/