Monthly Archives: August 2015

How to Calibrate an Autoclave?

Medical equipment is commonly sterilized in autoclaves. They are also used in microbiology laboratories. Autoclaves are available in many sizes. The smallest is a stovetop pressure cooker. Countertop models are used in dentist’s offices and small medical clinics. Large solid-state controlled autoclaves are common in laboratories and hospitals. All autoclaves have temperature and pressure gauges, as well as a timer. Quarterly calibration of the autoclave timer and gauges is important to insure that proper sterilization is occurring.

Vertical Autoclave

Vertical Autoclave

Timer Calibration

  • Set the timer on the autoclave for a full cycle. Hold the stopwatch and be prepared to click it.
  • Turn on the autoclave and the stopwatch simultaneously.
  • Repeat the process if the time and the stopwatch do not match.
  • If the autoclave timer differs from the stopwatch time by more than 30 seconds, post an appropriate correction factor directly on the autoclave.
  • Note the calibration results in a logbook.
  • Temperature Calibration
  • Place the maximum registering thermometer near the built-in temperature sensor.
  • Load and operate the autoclave as usual.
  • Record the maximum autoclave temperature according to the built-in temperature display.
  • Let the autoclave cool.
  • Remove the maximum registering thermometer from the autoclave and record the temperature reading in the logbook.
  • Record the discrepancy if the maximum temperature of the built-in display is different from the maximum temperature on the registering thermometer.
  • Post a correction factor on the autoclave control panel if the temperatures were different.
  • Pressure Calibration
  • Calibrate the timer and temperature gauges before these steps.
  •  Load and operate the autoclave as usual. When the temperature display shows 121 degrees Celsius, the pressure gauge should show 15 pounds per square inch (15 psi).
  •  Record the discrepancy if there is any.
  •  Mark the correct point for 15 psi on the cover of the gauge and post a correction factor on the autoclave if this is not true.
    Vertical Autoclave

    Vertical Autoclave

    More information about autoclave,please view the website: Teach You How to Use an Horizontal Cylindrical Autoclave

An horizontal cylindrical autoclave is the most effective way of sterilizing medical and scientific equipment. While simple boiling in water at normal pressure kills most microbes and spores, it won’t kill them all. An horizontal cylindrical autoclave pressurizes the water to 1 atmosphere or 15 pounds per square inch, which in turn raises the water’s boiling point. The horizontal cylindrical autoclave then heats the water until boiling its new, elevated boiling temperature, at which point it is hot enough to kill all forms of life and thus sterilize the apparatus or equipment inside within 15 minutes.


Horizontal Cylindrical Autoclave


  1. Twist the control knob for the drain valve on the horizontal cylindrical autoclave, usually located on the bottom front of the machine, to the closed position.
  2. Open the door on the horizontal cylindrical autoclave and add hot water in the bottom of this opening, filling it to just below the front reservoir lip. On most machines this will take about 1 gallon of water.
  3. Load the material to be sterilized into the shelf in the horizontal cylindrical autoclave. Liquids in bottles should be 3/4 or less full, with lids slightly loosened. Clean petri dishes should be loaded in top side up, contaminated petri dishes get loaded agar side down, and instruments should be rolled in brown paper, which is then stapled to secure it.
  4. Close the door of the horizontal cylindrical autoclave and press down on the long handle to latch it securely.
  5. Toggle the exhaust switch, located at the top middle of most machines, to slow exhaust if you are sterilizing materials that contain water, or fast exhaust for sterilizing apparatus.
  6. Twist the timer to select the appropriate length of sterilization time. For small-volume machines this is 15 minutes, for machines containing over 1 liter of volume this is 30 minutes, and for larger machines with 1 gallon or more of volume you should select a 60 minute cycle. This starts the machine.
  7. Wait until the timer and pressure indicator both read zero, and then open the door, taking care to keep your hands, arms and face away from the upper parts of the door where steam is emerging and might burn you.
  8. Remove sterilized items using sterile, heat-proof gloves. Pour any melted contaminated agar away immediately into a biohazard container, then rinse glassware with hot water.
  9. Twist the drain valve control on the horizontal cylindrical autoclave to the open position to empty the water.
    Horizontal Cylindrical Autoclave For Sale

    Horizontal Cylindrical Autoclave For Sale

    More information about horizontal cylindrical autoclave,please check the link:

Autoclave use and cycles Shard by

Table Top Autoclave Use

Autoclaves provide a physical method for disinfection and sterilization.They work with a combination of steam, pressure and time. Autoclaves operate at high and pressure in order to kill microorganisms and spores.

They are used to decontaminate certain biological waste and sterilize media, instruments and lab ware. Regulated medical waste that might contain bacteria, viruses and other biological material are recommended to be inactivated by autoclaving before disposal.

Table_Top_Autoclave_TS-CD20 24

Table Top Autoclave TS-CD-Bluestone Autoclave

Table Top Autoclave Cycles

The rate of exhaust will depend upon the nature of the load. Dry material can be treated in a fast exhaust cycle, while liquids and biological waste require slow exhaust to prevent boiling over of super-heated liquids.

More products information about sterilization, please refer:

table top combo 2

Table Top Autoclave manufacture

temperature of 121

Tips to Assure Effective Sterilization by Portable Autoclaves shared by

Failure to effectively sterilize an portable autoclave load can be due to either a mechanical problem with the equipment or operator error. Operator errors are generally due to insufficient steam circulation and penetration due to:

  • Autoclaving for too short of a time for the size and configuration of load
  • Using an autoclave bag that is too large for the portable autoclave
  • filling bags more than third fourth
  • Closing bags before autoclaving
  • Not having enough liquid in bottom of bag to create steam and force air out
  • Placing material in plastic container that does not allow efficient heat transfer
  • Placing material in plastic container with high sides that does not allow effective steam penetration
Portable Autoclave for sale-Bluestone Autoclave

Portable Autoclave-Bluestone Autoclave

Note: Do Not autoclave plastic unless it is rated as autoclavable. Do not exceed the temperature that the material is rated for. Melted plastic releases fumes, ruins autoclave surfaces and can clog chamber drains.

More products information about sterilization, please refer:

 Autoclave manufacture

Autoclave manufacture-Bluestone Autoclave

Many Advantages Of Autoclaves

Many Advantages Of  Autoclaves.

Autoclaves are instruments that are made to bear steam at a temperature of 121 degrees Celsius and pressure so as to sterilise them. The pressure and heat eliminates the bacteria, and other pathogens effectively so that autoclaves can be used extensively in medical facilities and laboratories for the preparation of life science application and culture media for microbiology, to sterilize medical instruments, glassware and to decontaminate harmful medical waste after disposal.


Autoclaves are the sterilization apparatus of choice in hospitals and laboratories. They are also used in dentistry, veterinary medicine, development and research of food production and pharmaceuticals facilities. Large and small autoclaves can be utilized anywhere considering the sterilization of the equipment is significant to ensuring the result of the process, the safety of public and person like in business which offer body piercing and tattooing services.

Autoclaves are also used in industrial applications where materials and parts require to be sterilized thoroughly during the process of production, this is comparatively less common in industries working with composite materials, specifically in the aerospace sector. Large sized autoclaves may be needed to accommodate some of the components because of their sheer size. Safety is a matter of great concern while using these devices.


More information about autoclaves,please refer to:


Need affordable Autoclave & Laboratory Equipment? Then browse

Need affordable Autoclave & Laboratory Equipment? Then browse

While selecting affordableautoclave and laboratory equipment the buyer or the dealer has to keep certain facts and aspects in mind and being one of the leading laboratory autoclaves manufacturers of quality laboratory equipments like fully automatic autoclave, sterilizers, vertical autoclave and many other medical instruments which have high functional and relevant values. In case you are searching for a reliable and high-quality autoclave for media preparation, Bluestone Autoclave assures you of providing you with the most reliable and best-quality equipments made out of reliable raw materials to ensure its longevity and functionality apart from all safety measures which the equipments meet successfully.


Products like vacuum autoclave manufactured by Bluestone Autoclave are highly preferred in the market. The company has earned its reputation and good will for not only supplying highly attributed and dependable medical instruments and equipments but also for meeting all standards and measures of safety, functionality and quality about the same. The medical instruments manufactured Bluestone Autoclave Co. are needed for various medical tests, diagnosis, sterilization and various other purposes in differing medical centers, hospitals, diagnosis centers, nursing homes and so on. As one of the leading laboratory equipment suppliers India, Bluestone Autoclave Co. can be highly relied upon by its clients and customers for their flawless and no-complain error-free supply of all kinds of medical instruments and equipments of various kinds and needs.


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