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News about Autoclave Sterilizer

About  Sterilizer

In today’s world, the need for infection control has never been greater.  The patients and the health care professionals have become more concerned about the transmission of pathogenic organism then ever before.  Controlling microbial contamination through sterilization has long been considered the most essential component of an infection control program.  The result of proper instrument sterilization is the protection of the patient, and the health care professional from various infection diseases. The focus on infection control has sharpened.

Since infections increase the severity of illness, complicate recovery, and prolong inpatient stays they raise the cost of care.  The ever growing expense of health care has focused providers’ attention on reducing costs.  Often this means re-sterilizing and re-using expensive plastic items that might otherwise be discarded. Instruments must be sterilized between usage. There are several types of sterilization equipment.  Steam sterilizers (autoclaves), dry heat sterilizers,  heated chemical vapor sterilizers, and gas sterilizers.


Dry Heat

The dry heat sterilizers employ higher temperatures to destroy microorganisms.  They are considered effective and safe for metal instruments because the process does not dull instrument edges or rust/corrode the instruments. There are two kinds of ‘Dry heat sterilizers’.  The traditional ‘toaster oven’ style that is slow and take an hour to sterilize at 320f (160c), or the COX Rapid Heat Sterilizer which is using a patented forced air and baffle system technology to sterilize in 6 minutes at 375f (190c).  CDC guidelines calls for weekly monitoring the working of the dry heat sterilizer by doing a weekly spore test.

Steam Sterilizer

Steam sterilizers will allow you to sterilize a wide range of materials.  Temperature, pressure, and time are the main factors of this process.  The higher the temperature, the greater the pressure and the shorter the sterilization cycle.  Maximum temperature will rise to 273f (133c).  Steam sterilization , however, may cause unprotected instruments to corrode and unprotected cutting edges to dull.  Furthermore, excess water in the steam can provide a portal for microorganism to penetrate wet instrument packages.  Steam sterilizers (autoclaves) are the most popular method of sterilization and are made and sold throughout the world.  There are several steam sterilizers sold in the USA, and many more through the rest of the world.  In the table top environment, chamber sizes ranges from 6″  (15.24 cm) diameter  x 14″ (35.56 cm) deep, to 15″ (38.1 cm) diameter x 26″ (66.04 cm) deep.  CDC guidelines calls for weekly monitoring the working of the steam sterilizer by doing a weekly spore test.
Heated Chemical Vapor Sterilizers

These type of sterilizers also offer relatively short cycle times.  Metal instruments can be processed with minimal rust or corrosion, and cutting edges remain sharp; however, instruments must be dried completely before processing.  The sterilizer requires a special solution;  and heat sensitive plastics may be destroyed.   In addition, the unit must be placed in a well ventilated area to diffuse the chemical odor. CDC guidelines calls for weekly monitoring the working of the chemical sterilizer by doing a weekly spore test.

Chemical Solution
Liquid disinfectants, such as glutaraldehyde, are widely used in health care facilities.  They have short, inexpensive disinfection cycles.  However, gIutaraldehyde can not be used for all medical materials.  Typical cycle times and concentrations only provide disinfecting, which can fail to kill resistant microorganisms.  The solutions are highly corrosive and toxic;  since they come in liquid form, they can not be used with barrier packaging.  The moment an instrument is removed from the liquid, its sterility is compromised.  Immersion in a liquid steriliant is not recommended because sterilization by liquid chemicals can not be monitored biologically.  In addition, instruments disinfected by liquids must be handled aseptically, rinsed in sterile water, and dried with a sterile towel.  Furthermore, instruments immersed in liquid sterilants are  not wrapped and, therefore, must be used immediately or stored in a sterile container, according to the American Dental Association Councils on Scientific Affairs and on Dental Practice’s “Infection Control Recommendations for the Dental Office and the Dental Laboratory,” presented at the 1996 OSAP Scientific Forum.

sterilizers VA-FJ

Glass Bead Sterilizers

Glass bead sterilizer have long been employed for instruments re-used on the same patient at a single appointment;  however, they are not suitable for terminal sterilization of instruments prior to re-use on other patients.  The reason they are not effective is that there isn’t a way to test the temperature throughout the cycle.  In fact, glass bead sterilizers are not FDA approved, and any new machines must receive pre market approval, according to Dr. Janie Fuller with the infection control devices branch of the U.S. Food & drug Administration.

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What is Sterilization?

Sterilization may be defined as the statistically complete destruction of all microorganisms including the most resistant bacteria and spores. This is a condition that is difficult to achieve and hard to prove. Whilst there are many chemicals, inorganic and organic, that kill microorganisms they may not be totally effective and can leave undesirable or toxic residues.

Ultraviolet and Ionising radiations are also effective biocides, disrupting or modifying the DNA to prevent replication, but Ultraviolet will not produce the effective results and easy validation that moist heat (steam) sterilization can provide. If sterility is an absolute requirement then today’s scientists turn, as their predecessors did, to steam.

Horizontal Cylindrical Steam Sterilization

Horizontal Cylindrical Steam Sterilization

Microorganisms tend to become more active as the temperature of their surroundings rises, – most, but not all, die at above 80oC. In the case of Prions the temperature and time requirements for deactivation are much higher. Steam molecules condense on cooler microorganisms, and transfer 2500 joules per gram of steam, very efficiently heating the microorganisms to the temperature at which they are destroyed. Other methods of heating suffer from the much lower heat transfer of hot dry gases and boundary layer effects, which can insulate and protect the microorganisms.

For maximum effect the Steam must be saturated, and this condition, and the temperature and pressure of the steam are easily monitored, facilitating proof that sterilization has occurred. By employing Steam Sterilization techniques a high level of sterility can be achieved and the most popular piece of equipment used in laboratories and hospitals is the steam sterilizer or autoclave.

Sterilization TS-DDV50

More information about Steam Sterilization,please check the website: teaches you how to autoclave a liquid

An autoclave machine uses pressurized steam to sterilize. The heat and steam in the autoclave force air out of the autoclave chamber and destroy microorganisms in liquid. Besides sterilization, an autoclave is used to decontaminate dangerous liquid waste before it is thrown away. In order to sterilize the liquid properly, the autoclave needs to be used properly. Using the wrong containers and heat and pressure programs can damage the autoclave.

Bluestone Desktop Autoclave

Bluestone Desktop Autoclave



Bluestone Desktop Autoclave


  1. Fill liquid containers half full with the liquid. Appropriate containers include polypropylene jars or tubes or laboratory-grade glassware; they can withstand the temperatures and pressure of the autoclave process without breaking. Place each container’s lid on the container loosely. If the lid is a screw cap, then tighten the cap only half way. In place of a cap, you cover a container’s opening with aluminum foil. Tape a piece of autoclave tape to each container. At the end of the desktop autoclave process, the tape will reveal whether or not the autoclave machine reached the proper temperature.
  2. Place the covered liquid containers in a secondary container, such as a stainless steel or polypropylene pan, that is at least 5 inches deep. You may place more than one container in a pan. The secondary container catches material that boils out of liquid containers that were filled too high. It also prevents containers or other items from falling to the bottom of the desktop autoclave and making a mess.
  3. Arrange the liquid containers in the pan so that space is between the containers. The space is necessary, allowing steam to circulate around each item.
  4. Select the liquid cycle on the desktop autoclave. The liquid cycle uses a slow exhaust, which helps to prevent liquid from boiling out of the containers. Set the time for the liquid cycle. Liquid containers that are large take longer to autoclave than containers that are small. For example, 1L of liquid in a 2L bottle requires longer time in an autoclave than 250mL of liquid in four 500mL flasks. Check the autoclave instructions for the appropriate autoclave time.
  5. Place the pans holding the containers in the desktop autoclave. Close the autoclave door, rotating its handle clockwise until the door seals tightly. If the door does not seal correctly, then steam will leak, and the autoclave will not reach the necessary temperature or pressure.
  6. Start the desktop autoclave, and allow the cycle to finish. Before opening the autoclave chamber, verify that the autoclave pressure is zero.
  7. Remove the pans from the autoclave with heat-insulated gloves. The containers still may be hot. Allow the liquid to cool completely before use.
table top combo 2

Bluestone Desktop Autoclave 2015 New Arriving

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How to clean Portable Autoclave?

Portable Autoclaves are used for cleaning and sterilizing medical and cosmetic instruments, but that doesn’t keep them from needing a good cleaning, too, every once in awhile. The best way to clean an portable autoclave is to perform light cleaning on a daily basis, with heavier cleaning reserved for a weekly or monthly basis. Make sure to use only those cleaning supplies and accessories approved by the autoclave manufacturer to avoid damage to the autoclave.

Portable Autoclave PA-ND - Bluestone Autoclave

Portable Autoclave PA-ND – Bluestone Autoclave


  1. Get in the habit of wearing latex gloves while you clean the portable autoclave to help protect your hands. Even though all matter inside the autoclave should be sterile, this is still a good habit.
  2. Dust the outside of the portable autoclave with a dry rag every day. Rinse the inside with clean water to remove any debris, and pluck any matter out of the drain. You may need tweezers to help remove matter from the autoclave drain.
  3. Clean and descale the portable autoclave drain at least once a week with a manufacturer-approved descaler like All-Met. Fill a spray bottle with one part All-Met to fifteen parts clean water, spray inside the autoclave and scrub with an autoclave cleaning brush.
  4. Rinse the portable autoclave thoroughly after each cleaning; always follow all instructions on the Material Safety Data Sheets (MSDS) sheets for cleaning products, which should be on file in your medical office. Leave the drain on the autoclave open, so that the rinse water runs all the way through it.

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portable combo

                          Bluestone Portable Autoclave

Types of Table Top Autoclaves

An Table Top autoclave is a device that is used to sterilize medical, beautician and other equipment such as tattooing and piercing paraphernalia. The basic function of the machine is to kill bacteria. This is done through application of extreme heat, steam and pressure to the articles placed in the sterilization chamber. This is accomplished by the removal of air from the sterilization chamber to create a vacuum, then superheated steam is applied. There are several types of autoclave in use.

Table Top Autoclave-Bluestone Autoclave

Table Top Autoclave-Bluestone Autoclave

Downward Displacement

  • The downward displacement autoclave is also referred to as a gravity displacement unit. This is because of the method of air removal in the sterilization chamber. A heating element is submerged in a pool of water, which, when heated, becomes steam. As steam is lighter than air, it forces the air in the sterilization chamber downward and out through a drain hole. Once the temperature in the sterilization chamber is sufficient, the drain hole is closed automatically and the sterilization process begins. It is very common one in table top autoclave.

Positive Pressure Displacement

  • A positive pressure displacement autoclave is an improvement upon the design of a downward displacement unit. Steam is created in a second, separate chamber and held until the proper amount to displace all of the air in the sterilization chamber is accumulated. The steam is then released into the sterilization chamber in a pressurized blast, forcing the air out through the drain hole and starting the sterilization process. This has the effect of a more accurate displacement of air than a downward displacement unit can achieve.

Negative Pressure Displacement

  • A negative pressure displacement autoclave is one of the most accurate types of unit available. Once the sterilization chamber door is closed, a vacuum pump removes the air. Steam is created in a second, separate chamber. Once the air has been completely removed from the sterilization chamber, the steam is then released into the sterilization chamber in a pressurized blast much like that of a positive pressure displacement unit. The negative pressure displacement unit is able to achieve a high “Sterility Assurance Level” (SAL), but the system can be quite large and costly.

Triple Vacuum Autoclave

  • A triple vacuum autoclave is set up in a similar fashion to a negative pressure displacement unit in that there is a vacuum pump to remove air from the sterilization chamber and steam is created in a second, separate chamber or unit. The process begins by the vacuum removing the air, then a pulse of steam. This is repeated three times, hence the name “triple vacuum” autoclave. This type of autoclave is suitable for all types of instruments and is very versatile.

Type “N” vs. Type “B”

  • Each table top autoclave can be classified as a type “N” unit or a type “B” unit. Type “N” units do not use a vacuum to remove air from the sterilization chamber, whereas type “B” units do use a vacuum pump. The difference in operation means type “N” autoclaves are suitable for a specific type of load–for solid, unwrapped instruments. Type “B” autoclaves can be used on wrapped and hollow instruments, which means a piece of equipment can be sterilized now for use later.
  • small autoclave sterilizer

    Class N Table Top Autoclavce – Bluestone Autoclave

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When purchasing a table top autoclave there are many factors to consider.

1. Do you need a manual unit or a fully automatic table top autoclave and what is the difference?

Table Top Autoclave

A manual table top autoclave requires the operator to manipulate the machine through each cycle. It is a multi step process over the course of an hour. Step one is to turn a valve, knob or lever (depending on autoclave model) to fill the table top autoclave chamber to the water fill line. You then load the autoclave with the instruments to be sterilized. Next, secure the door and set the timer so the autoclave can begin the heat up process. Once the machine has reached sterilization temperature and pressure the operator then sets the sterilization timer. The table top autoclave will now begin the count down. A buzzer will ring and now it is time to vent the unit. When the autoclave is properly exhausted the operator then opens the door to commence the drying cycle.

Most busy clinics utilize a fully automatic or semi-automatic autoclave. These autoclaves are designed to load, set and forget. You simply load it, close the door, push a button and the autoclave automatically fills, sterilizes, vents and dries without operator assistance. The convenience factor many times far out weighs the additional cost.

2. What is the cost of ownership?

table top autoclave

A well maintained table top autoclave is not expensive to own. It will require distilled water that can be purchased at your local retail or grocery store and a regular cleaning routine. Each model uses a different type of autoclave cleaning solution. It is a fairly simple process. The key is to do this on a regular consistent schedule. The door gasket will need to be replaced every 8 to 14 months depending on hours of use.


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Parts & Functions of an Desktop Autoclave

An Desktop autoclave, whether for use in a medical office, body art salon or beauty salon, is essentially a pressure cooker used to kill bacteria and viruses. By virtue of pressure and superheated steam, an autoclave will destroy any micro-organisms on metal objects used in these settings, allowing for the reuse of metal objects without the risk of infection. Medical and research laboratories also use autoclaves sterilize culture media before growing bacteria for experiments. Autoclaves have four basic parts.

Desktop Autoclave Sterilizer - Bluestone Autoclave

Desktop Autoclave Sterilizer – Bluestone Autoclave

Water Intake
An autoclave needs water to make the steam used for sterilization. Smaller Desktop autoclave will have a reservoir that requires you to refill it before use, as with a coffeemaker. Large standing models, such as those used in laboratories, will have a water intake hookup or hose, allowing the user to pump water directly into the machine.

The chamber is the space where the user places items to sterilize. In the chamber are wire racks, like those in a dishwasher, which will hold various items upright or lying down and allow for steam penetration from all angles.

Control Panel
The control panel allows the user to customize the autoclaving process. Some materials can withstand higher temperatures, while some must be autoclaved at lower temperatures for longer time. The heat, pressure and time will vary with each type of item autoclaved, creating the need for a control panel. Some Desktop autoclaves will include automated settings for specific autoclaving functions; these models will have “fast buttons” for those settings.

Vacuum Desktop Autoclaves must have an air pump system to remove the oxygen in the chamber and create a vacuum which then fills with pressurized steam created from the water in the reservoir. The water becomes heated either via a heating element inside the water reservoir or a heat-generating mechanism that completely surrounds the reservoir.

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table top combo 2

Desktop Type Autoclave – Bluestone Autoclave

How to use the right autoclave

Autoclave could be a science laboratory instrument that may even be in hand by individual and manufacturer. In laboratory this instrument is employed to sterilize risky microorganisms et al.

Sterilization suggests that the destruction of organism life as well as spores, viruses, and others. This instrument should be checked often to understand whether or not it’s operating properly or not. If the steam doesn’t directly contact the risky materials, the microorganisms don’t seem to be destroyed by the autoclaving method. To validate the effectiveness of sterilization method you got to perform vials take a look at. you wish to perform this internal control monthly. you furthermore may got to clean the unit often reckoning on the things that are autoclaved

Pulse Vacuum Autoclave

Horizontal large Autoclave HA-BVD

All things that square measure getting to be sterilized should be placed within the receptacle. The receptacle should be ready to handle the things from boiling over.. check that that the things that you just square measure getting to sterilize square measure autoclaveable. Pay special attention to risky liquids. The vessel should be doubly larger than the liquid within. this can be to forestall spilling once the liquid is boiling within the chamber.

The caps should be unsnarled to forestall vessel breakage. you have got to place garbage within autoclave luggage and trays of five in. sides. Autoclave is associate instrument that encompasses a risk in usage. This instrument generates plenty of energy within the sterilization method. There square measure several prospects of injury that may happen to the user. to forestall the attainable injury caused by the wrong usage of this instrument, you have got to follow the steps below.

  1. Wear apron, gloves, and glasses to handle hot vessels.
  2. don’t forget to shut it off and undo the cable before removing the materials from the chamber.
  3. Let the materials cool before removing them from the autoclave vessel.
  4. rigorously take away the materials from the autoclave.

Beginner level of autoclave user ought to find out about however it operates before setting out to use this instrument. totally different completely different kind of autoclave may need different instruction in victimization it.

albeit you’re an expert user, you have got to browse the manufacturer’s instruction for the right usage of the autoclave.


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How to Calibrate an Autoclave?

Medical equipment is commonly sterilized in autoclaves. They are also used in microbiology laboratories. Autoclaves are available in many sizes. The smallest is a stovetop pressure cooker. Countertop models are used in dentist’s offices and small medical clinics. Large solid-state controlled autoclaves are common in laboratories and hospitals. All autoclaves have temperature and pressure gauges, as well as a timer. Quarterly calibration of the autoclave timer and gauges is important to insure that proper sterilization is occurring.

Vertical Autoclave

Vertical Autoclave

Timer Calibration

  • Set the timer on the autoclave for a full cycle. Hold the stopwatch and be prepared to click it.
  • Turn on the autoclave and the stopwatch simultaneously.
  • Repeat the process if the time and the stopwatch do not match.
  • If the autoclave timer differs from the stopwatch time by more than 30 seconds, post an appropriate correction factor directly on the autoclave.
  • Note the calibration results in a logbook.
  • Temperature Calibration
  • Place the maximum registering thermometer near the built-in temperature sensor.
  • Load and operate the autoclave as usual.
  • Record the maximum autoclave temperature according to the built-in temperature display.
  • Let the autoclave cool.
  • Remove the maximum registering thermometer from the autoclave and record the temperature reading in the logbook.
  • Record the discrepancy if the maximum temperature of the built-in display is different from the maximum temperature on the registering thermometer.
  • Post a correction factor on the autoclave control panel if the temperatures were different.
  • Pressure Calibration
  • Calibrate the timer and temperature gauges before these steps.
  •  Load and operate the autoclave as usual. When the temperature display shows 121 degrees Celsius, the pressure gauge should show 15 pounds per square inch (15 psi).
  •  Record the discrepancy if there is any.
  •  Mark the correct point for 15 psi on the cover of the gauge and post a correction factor on the autoclave if this is not true.
    Vertical Autoclave

    Vertical Autoclave

    More information about autoclave,please view the website: Teach You How to Use an Horizontal Cylindrical Autoclave

An horizontal cylindrical autoclave is the most effective way of sterilizing medical and scientific equipment. While simple boiling in water at normal pressure kills most microbes and spores, it won’t kill them all. An horizontal cylindrical autoclave pressurizes the water to 1 atmosphere or 15 pounds per square inch, which in turn raises the water’s boiling point. The horizontal cylindrical autoclave then heats the water until boiling its new, elevated boiling temperature, at which point it is hot enough to kill all forms of life and thus sterilize the apparatus or equipment inside within 15 minutes.


Horizontal Cylindrical Autoclave


  1. Twist the control knob for the drain valve on the horizontal cylindrical autoclave, usually located on the bottom front of the machine, to the closed position.
  2. Open the door on the horizontal cylindrical autoclave and add hot water in the bottom of this opening, filling it to just below the front reservoir lip. On most machines this will take about 1 gallon of water.
  3. Load the material to be sterilized into the shelf in the horizontal cylindrical autoclave. Liquids in bottles should be 3/4 or less full, with lids slightly loosened. Clean petri dishes should be loaded in top side up, contaminated petri dishes get loaded agar side down, and instruments should be rolled in brown paper, which is then stapled to secure it.
  4. Close the door of the horizontal cylindrical autoclave and press down on the long handle to latch it securely.
  5. Toggle the exhaust switch, located at the top middle of most machines, to slow exhaust if you are sterilizing materials that contain water, or fast exhaust for sterilizing apparatus.
  6. Twist the timer to select the appropriate length of sterilization time. For small-volume machines this is 15 minutes, for machines containing over 1 liter of volume this is 30 minutes, and for larger machines with 1 gallon or more of volume you should select a 60 minute cycle. This starts the machine.
  7. Wait until the timer and pressure indicator both read zero, and then open the door, taking care to keep your hands, arms and face away from the upper parts of the door where steam is emerging and might burn you.
  8. Remove sterilized items using sterile, heat-proof gloves. Pour any melted contaminated agar away immediately into a biohazard container, then rinse glassware with hot water.
  9. Twist the drain valve control on the horizontal cylindrical autoclave to the open position to empty the water.
    Horizontal Cylindrical Autoclave For Sale

    Horizontal Cylindrical Autoclave For Sale

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