How to Calibrate an Autoclave?

Medical equipment is commonly sterilized in autoclaves. They are also used in microbiology laboratories. Autoclaves are available in many sizes. The smallest is a stovetop pressure cooker. Countertop models are used in dentist’s offices and small medical clinics. Large solid-state controlled autoclaves are common in laboratories and hospitals. All autoclaves have temperature and pressure gauges, as well as a timer. Quarterly calibration of the autoclave timer and gauges is important to insure that proper sterilization is occurring.

Vertical Autoclave

Vertical Autoclave

Timer Calibration

  • Set the timer on the autoclave for a full cycle. Hold the stopwatch and be prepared to click it.
  • Turn on the autoclave and the stopwatch simultaneously.
  • Repeat the process if the time and the stopwatch do not match.
  • If the autoclave timer differs from the stopwatch time by more than 30 seconds, post an appropriate correction factor directly on the autoclave.
  • Note the calibration results in a logbook.
  • Temperature Calibration
  • Place the maximum registering thermometer near the built-in temperature sensor.
  • Load and operate the autoclave as usual.
  • Record the maximum autoclave temperature according to the built-in temperature display.
  • Let the autoclave cool.
  • Remove the maximum registering thermometer from the autoclave and record the temperature reading in the logbook.
  • Record the discrepancy if the maximum temperature of the built-in display is different from the maximum temperature on the registering thermometer.
  • Post a correction factor on the autoclave control panel if the temperatures were different.
  • Pressure Calibration
  • Calibrate the timer and temperature gauges before these steps.
  •  Load and operate the autoclave as usual. When the temperature display shows 121 degrees Celsius, the pressure gauge should show 15 pounds per square inch (15 psi).
  •  Record the discrepancy if there is any.
  •  Mark the correct point for 15 psi on the cover of the gauge and post a correction factor on the autoclave if this is not true.
    Vertical Autoclave

    Vertical Autoclave

    More information about autoclave,please view the website: Teach You How to Use an Horizontal Cylindrical Autoclave

An horizontal cylindrical autoclave is the most effective way of sterilizing medical and scientific equipment. While simple boiling in water at normal pressure kills most microbes and spores, it won’t kill them all. An horizontal cylindrical autoclave pressurizes the water to 1 atmosphere or 15 pounds per square inch, which in turn raises the water’s boiling point. The horizontal cylindrical autoclave then heats the water until boiling its new, elevated boiling temperature, at which point it is hot enough to kill all forms of life and thus sterilize the apparatus or equipment inside within 15 minutes.


Horizontal Cylindrical Autoclave


  1. Twist the control knob for the drain valve on the horizontal cylindrical autoclave, usually located on the bottom front of the machine, to the closed position.
  2. Open the door on the horizontal cylindrical autoclave and add hot water in the bottom of this opening, filling it to just below the front reservoir lip. On most machines this will take about 1 gallon of water.
  3. Load the material to be sterilized into the shelf in the horizontal cylindrical autoclave. Liquids in bottles should be 3/4 or less full, with lids slightly loosened. Clean petri dishes should be loaded in top side up, contaminated petri dishes get loaded agar side down, and instruments should be rolled in brown paper, which is then stapled to secure it.
  4. Close the door of the horizontal cylindrical autoclave and press down on the long handle to latch it securely.
  5. Toggle the exhaust switch, located at the top middle of most machines, to slow exhaust if you are sterilizing materials that contain water, or fast exhaust for sterilizing apparatus.
  6. Twist the timer to select the appropriate length of sterilization time. For small-volume machines this is 15 minutes, for machines containing over 1 liter of volume this is 30 minutes, and for larger machines with 1 gallon or more of volume you should select a 60 minute cycle. This starts the machine.
  7. Wait until the timer and pressure indicator both read zero, and then open the door, taking care to keep your hands, arms and face away from the upper parts of the door where steam is emerging and might burn you.
  8. Remove sterilized items using sterile, heat-proof gloves. Pour any melted contaminated agar away immediately into a biohazard container, then rinse glassware with hot water.
  9. Twist the drain valve control on the horizontal cylindrical autoclave to the open position to empty the water.
    Horizontal Cylindrical Autoclave For Sale

    Horizontal Cylindrical Autoclave For Sale

    More information about horizontal cylindrical autoclave,please check the link:

Autoclave use and cycles Shard by

Table Top Autoclave Use

Autoclaves provide a physical method for disinfection and sterilization.They work with a combination of steam, pressure and time. Autoclaves operate at high and pressure in order to kill microorganisms and spores.

They are used to decontaminate certain biological waste and sterilize media, instruments and lab ware. Regulated medical waste that might contain bacteria, viruses and other biological material are recommended to be inactivated by autoclaving before disposal.

Table_Top_Autoclave_TS-CD20 24

Table Top Autoclave TS-CD-Bluestone Autoclave

Table Top Autoclave Cycles

The rate of exhaust will depend upon the nature of the load. Dry material can be treated in a fast exhaust cycle, while liquids and biological waste require slow exhaust to prevent boiling over of super-heated liquids.

More products information about sterilization, please refer:

table top combo 2

Table Top Autoclave manufacture

temperature of 121

Tips to Assure Effective Sterilization by Portable Autoclaves shared by

Failure to effectively sterilize an portable autoclave load can be due to either a mechanical problem with the equipment or operator error. Operator errors are generally due to insufficient steam circulation and penetration due to:

  • Autoclaving for too short of a time for the size and configuration of load
  • Using an autoclave bag that is too large for the portable autoclave
  • filling bags more than third fourth
  • Closing bags before autoclaving
  • Not having enough liquid in bottom of bag to create steam and force air out
  • Placing material in plastic container that does not allow efficient heat transfer
  • Placing material in plastic container with high sides that does not allow effective steam penetration
Portable Autoclave for sale-Bluestone Autoclave

Portable Autoclave-Bluestone Autoclave

Note: Do Not autoclave plastic unless it is rated as autoclavable. Do not exceed the temperature that the material is rated for. Melted plastic releases fumes, ruins autoclave surfaces and can clog chamber drains.

More products information about sterilization, please refer:

 Autoclave manufacture

Autoclave manufacture-Bluestone Autoclave

Many Advantages Of Autoclaves

Many Advantages Of  Autoclaves.

Autoclaves are instruments that are made to bear steam at a temperature of 121 degrees Celsius and pressure so as to sterilise them. The pressure and heat eliminates the bacteria, and other pathogens effectively so that autoclaves can be used extensively in medical facilities and laboratories for the preparation of life science application and culture media for microbiology, to sterilize medical instruments, glassware and to decontaminate harmful medical waste after disposal.


Autoclaves are the sterilization apparatus of choice in hospitals and laboratories. They are also used in dentistry, veterinary medicine, development and research of food production and pharmaceuticals facilities. Large and small autoclaves can be utilized anywhere considering the sterilization of the equipment is significant to ensuring the result of the process, the safety of public and person like in business which offer body piercing and tattooing services.

Autoclaves are also used in industrial applications where materials and parts require to be sterilized thoroughly during the process of production, this is comparatively less common in industries working with composite materials, specifically in the aerospace sector. Large sized autoclaves may be needed to accommodate some of the components because of their sheer size. Safety is a matter of great concern while using these devices.


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Need affordable Autoclave & Laboratory Equipment? Then browse

Need affordable Autoclave & Laboratory Equipment? Then browse

While selecting affordableautoclave and laboratory equipment the buyer or the dealer has to keep certain facts and aspects in mind and being one of the leading laboratory autoclaves manufacturers of quality laboratory equipments like fully automatic autoclave, sterilizers, vertical autoclave and many other medical instruments which have high functional and relevant values. In case you are searching for a reliable and high-quality autoclave for media preparation, Bluestone Autoclave assures you of providing you with the most reliable and best-quality equipments made out of reliable raw materials to ensure its longevity and functionality apart from all safety measures which the equipments meet successfully.


Products like vacuum autoclave manufactured by Bluestone Autoclave are highly preferred in the market. The company has earned its reputation and good will for not only supplying highly attributed and dependable medical instruments and equipments but also for meeting all standards and measures of safety, functionality and quality about the same. The medical instruments manufactured Bluestone Autoclave Co. are needed for various medical tests, diagnosis, sterilization and various other purposes in differing medical centers, hospitals, diagnosis centers, nursing homes and so on. As one of the leading laboratory equipment suppliers India, Bluestone Autoclave Co. can be highly relied upon by its clients and customers for their flawless and no-complain error-free supply of all kinds of medical instruments and equipments of various kinds and needs.


Follow us on: shares the tips of caring for Table Top Autoclave or Steam Sterilizer

Caring for your Table Top Autoclave  is very important and can be easily overlooked. We often see this step neglected which can cause high and frequent repair costs. A lack of maintenance will also lead to deterioration and excessive wear of the contents being sterilized.  Let go over a few points to make caring for your autoclave or steam sterilizer easier for you.

Table Top Autoclave TS-B24_Bluestone Autoclave

Table Top Autoclave TS-B24_Bluestone Autoclave


1) It is very important that the contents being sterilized are clean and free from debris, blood and organic tissue. Otherwise the instruments or sterilizer may become damaged.

2) Inspect the  door gasket of  table top autoclave for excessive wear or cracking. The gasket and mating surface should be cleaned with a mild detergent and using a cloth or sponge.

3) When running a sterilization cycle it is very important to not overload the sterilizer trays because it will cause inadequate sterilization and drying.

Table Top Autoclave TS-B24_ Bluestone Autoclave

Table Top Autoclave TS-B24_ Bluestone Autoclave


1) Keeping your Table top autoclave clean is one of the most important points. Be sure to clean the trays and rack with a Non-Scratch Scour Pad using a mild non-abrasive detergent.  Always rinse the instrument well and be sure to NOT USE steel wool, wire brush, or bleach.

2) Completely drain the water from the reservoir and replenish with fresh distilled water.


1) Clean the chamber and flush lines of table top autoclave  using the recommended autoclave cleaner following the cleaner’s instructions. Normally you will need 2 gallons of distilled water to complete this job.

2) Inspect the cord and plug for overheating and excessive wear—this could be a fire hazard. If this is the case, the power cord should be replaced. Some power cords are quick disconnect and easily replaced. If not, the replacement will need to be done by a certified repair company.


On a yearly basis you should have your table top autoclave inspected, cleaned thoroughly, tested and calibrated by autoclave technicians.

More products information about sterilization, please refer:

Autoclave shares the Autoclaving Procedures for Horizontal Autoclave

Horizontal Autoclaves use pressurized steam to destroy microorganisms, and are the most dependable systems available for the decontamination of laboratory waste and the sterilization of laboratory glassware, media, and reagents. For efficient heat transfer, steam must flush the air out of the autoclave chamber. Before using the autoclave, check the drain screen at the bottom of the chamber and clean if blocked. If the sieve is blocked with debris, a layer of air may form at the bottom of the autoclave, preventing efficient operation.

Horizontal Autoclave HA-BA

Horizontal Autoclave HA-BA_Bluestone Autoclave

Container Selection-Bluestone Horizontal Autoclave

  • Polypropylene bags. Commonly called biohazard or autoclave bags, these bags are tear resistant, but can be punctured or burst in the autoclave. Therefore, place bags in a rigid container during autoclaving. Bags are available in a variety of sizes, and some are printed with an indicator that changes color when processed.Polypropylene bags are impermeable to steam, and for this reason should not be twisted and taped shut, but gathered loosely at the top and secured with a large rubber band or autoclave tape. This will create an opening through which steam can penetrate.
  • Polypropylene containers and pans. Polypropylene is a plastic capable of withstanding autoclaving, but resistant to heat transfer. Therefore, materials contained in a polypropylene pan will take longer to autoclave than the same materials in a stainless steel pan. To decrease the time required to sterilize material in these containers,
    • remove the lid (if applicable).
    • turn the container on its side when possible.
    • select the container with the lowest sides and widest diameter possible for the autoclave.
  • Stainless steel containers and pans. Stainless steel is a good conductor of heat and is less likely to increase sterilizing time, though is more expensive than polypropylene.

Preparation and Loading of Materials-Bluestone Horizontal Autoclave

  • Fill liquid containers only half full.
  • Loosen caps or use vented closures.
  • Always put bags of biological waste into pans to catch spills.
  • Position biohazard bags on their sides, with the bag neck taped loosely.
  • Leave space between items to allow steam circulation.
  • Household dishpans melt in the autoclave. Use autoclavable polypropylene or stainless steel pans.
Loading chamber_Horizontal Autoclave HA-BC

Loading chamber_Horizontal Autoclave HA-BC

Cycle Selection-Bluestone Horizontal Autoclave

  • Use liquid cycle (slow exhaust) when autoclaving liquids, to prevent contents from boiling over.
  • Select fast exhaust cycle for glassware.
  • Use fast exhaust and dry cycle for wrapped items.

Time Selection-Bluestone Horizontal Autoclave

  • Take into account the size of the articles to be autoclaved. A 2-liter flask containing 1 liter of liquid takes longer to sterilize than four 500 mL flasks each containing 250 mL of liquid.
  • Material with a high insulating capacity (animal bedding, high sided polypropylene containers) increases the time needed for the load to reach sterilizing temperatures.
  • Autoclave bags containing biological waste should be autoclaved for 50 minutes to assure decontamination.

Removing the Load-Bluestone Horizontal Autoclave

  • Check that the chamber pressure is zero.
  • Wear lab coat, eye protection, heat insulating gloves, and closed-toe shoes.
  • Stand behind door when opening it.
  • Slowly open door only a crack. Beware of rush of steam.
  • After the slow exhaust cycle, open autoclave door and allow liquids to cool for 20 minutes before removing.

More production information, please visit our website:

What Steam Generation Options are available? Bluestone Autoclave

There are several different steam generator options available depending on the size of autoclave. Take our Horizontal autoclave and Table Top Autoclave as an example. As a general rule, autoclaves within 100 litres are available as ‘Direct Steam’ models at no extra cost. Most autoclaves over 800 litres require an external stand alone generator due to the power required to produce the levels of steam necessary for sterilization. If you require a specific configuration, then please Contact us ( for further information.

Heaters in Chamber Models

Heaters in chamber is the standard configuration fitted to all circular chambered  Bluestone autoclaves and Economy range models. On standard machines, water is manually poured by the user directly into the chamber before each cycle begins. As the heater element(s) reach temperature, the water in the chamber boils to produce steam. An Autofill option is available to allow automatic water filling to take place so that the autoclave does not need to be manually filled between cycles. This option is not suitable for sterilization cycles where drying is required for porous loads/fabrics.


Heaters in chamber Bluestone Autoclave


Table Top Autoclave TS-AJ-Blue stone Autoclave

Integral Steam Generator

The integral steam generator option removes the heating elements from the chamber and replaces them with an integral generator for improved control over steam production. ‘Autofill’ is also fitted as standard to the steam generator; eliminating the need to manually refill the chamber between cycles. A mains water supply enters the steam generator, where it is heated and converted to steam. This then enters the chamber directly from the steam generator as and when it is required. This option is available throughout the range on autoclaves 100 litres and above.

Integral Steam Generator

Integral Steam Generator-Bluestone Autoclave

Horizontal Autocalve HA-BC

Horizontal Autoclave HA-BC-Bluestone Autoclave

Direct Steam/External Steam Generators

The direct steam option is intended for sites which already have a steam supply, or for larger autoclaves that require a powerful stand alone steam generator. An external steam feed enters the unit as per the illustrated diagram. This option is available on Bluestone Autoclave from 800-2000litres and above. More production information, please visit our website:

Horizontal large Autoclave HA-BVD

Horizontal large Autoclave HA-BVD

Horizontal large Autoclave HA-BVD

Horizontal large Autoclave HA-BVD

Autoclaves are instruments that are used to eradicate all forms if microbes which includes fungi, viruses and bacteria. These instruments are broadly used in chiropody, dentistry, body piercing, tattooing, and mycology. There are different types of technologies that are used in the process of sterilization. Technologies such as chemical sterilization, heating and ultra-violet light are used in the process of sterilization.
High pressure autoclaves can reach the desired temperature that is higher than the boiling point which will not only kill bacteria but also bacterial spores. The basic function of autoclaves in laboratories is to make items such as glass ware and equipments used in surgery, free from infection and contamination. There can be different types of autoclaves with different features.
They are based on a principle that the boiling point of water tends to increase when it is below pressure. For instance, at 15 pounds of pressure per inch, the boiling water definitely increases from hundred degrees Celsius to say one hundred and twenty degrees. At this level of temperature, all forms of life can be killed within fifteen minutes.

High Pressure Autoclave For Sale

Equipments used in laboratory and hospitals require high pressure sterilizer and these devices are autoclaves. These autoclaves are computer controlled and are extremely energy efficient .They are even provided with the facility to program recording interval with several other options to suit individual requirements. The system even offers the feature to adjust or select any baud rate for any serial communication port.
As mentioned above, there are technologies that are used in the process of sterilization. It is not always necessary, that heating would be the best option to perform this task. There are cases, in which heating damages the materials and these materials can be in the form of plastics, rubber optics and fiber. However, when heating damages these materials, chemical sterilization is used, as these materials need external venting systems that indeed remove all the chemical agents from the sterilizer. And, ultra violet rays produce UV rays that create a lethal effect on unwanted diseases, which completely destroys microbes like bacteria, fungi, pathogens, yeast and protozoa. Apart from these technologies, heating is considered as the most suitable form of sterilization.
In the entire process, time plays a critical role, as it is important to find out what time is needed to sterilize an equipment. Another important factor, that is extremely important is, safety. It is significant that the personnel should wear proper equipment while loading these devices.

More information about high pressure autoclaves,Please refer:

High pressure autoclave for sale