Want to Know Different Kinds of Sterilizing Equipments?

Want to Know Different Kinds of Sterilizing Equipments?

1 ) , steam sterilization equipment
High-pressure steam sterilization equipment is in use the longest history , the most extensive application of a sterile manner , is still widely used today . But it has been carried out in the traditional way has been improved, introduced a way of pulsating vacuum steam sterilization equipment , improve the quality of sterilization and shorten the sterilization cycle.
2 ) , Dry Heat Sterilizer
Dry heat sterilization its place rapidly in the field of sterilization , dry heat sterilizer narrower , so the equipment has certain limitations. However, the dry heat sterilizer capable of sterilizing powder -like objects other sterilization equipment is difficult to achieve .

3 ) , microwave sterilizer
With the need for rapid sterilization in recent years, so the rise in microwave sterilization , and confirmed its unique characteristics, but also belong to the immaturity of microwave sterilization technique needs further research and development.Stay tuned on http://www.autoclavesale.com/ to get the latest news.

4 ) , ethylene oxide sterilization equipment
With the popularity of ethylene oxide sterilization of disposable products and became the second largest major sterilization equipment , which is characterized by low temperatures , humidity , penetration , a feature that is suitable for disposable products manufacturer final product centralized sterilization , and therefore is widely used in the domestic production of disposable medical devices companies . But in recent years have begun to report foreign literature ethylene oxide sterilization equipment is limited to the formulation used , mainly due to: the toxic ethylene oxide , the operator , the environment affects both , there is a reference to the ethylene alkyl can lead to cancer. So for ethylene oxide sterilization needs further research.

5 ) hydrogen peroxide plasma sterilizer
Hydrogen peroxide ( hydrogen peroxide ) is itself a disinfectant , but as sterilizing media sterilization equipment began in recent years . From the formula of hydrogen peroxide can completely remove toxic or harmful suspects , and the sterilization process is temperature, humidity , fast , fully in line with the future development direction of sterilization equipment , is likely to become ethylene oxide sterilization equipment alternatives.

6 ) , the ozone sterilizer
Ozone air sterilization sterilization sterilization is also popular in recent years, the rise of technology, mainly used in operating rooms and other environments as an alternative to the existing chemical fumigation , ultraviolet radiation and other methods .

The Fundamental Difference Between Moist Heat Sterilization And Dry Heat Sterilization

The Fundamental Difference Between Moist Heat Sterilization And Dry Heat Sterilization

Moist heat and dry heat factors are the use of heat to kill microorganisms physical sterilization methods , but their different sterilization mechanism , i.e. primarily through the moist heat coagulation of protein caused the death of microorganisms , dry heat dehydration by law drying the protein oxidation, denaturation, carbonization and electrolyte concentrate poisoning leaving microbial death .

Their operating characteristics are different, the fundamental difference is that when the sterilization treatment, environmental and microbial cells heated humidity levels differ: ” heat ” heating means heating the internal microbes in this environment are in the humidity and heat saturation in this case microorganisms and pure water or saturated steam was equilibrium ; Check more news on http://www.autoclavesale.com/ site .while ” dry heat ” heating , heating environment is used to refer to water or enough to reach saturation under heat and humidity , the humidity level can be zero , or just below saturation.

Dry heat sterilization on poor penetration dish plus iron , it is not recommended to be dry heat sterilized Petri dishes , and a large -capacity glass containers are not suitable for moist heat sterilization . Overall : 500ml large capacity of more than thicker glass and glass bottles are not suitable for moist heat sterilization , dry heat sterilization can be used , the effect is more ideal , but the dish is moist heat sterilization is better, small capacity glass bottles and glass bottles thinner two methods can be used.

Dry Heat Sterilizer Sterilization Principle

Dry Heat Sterilizer Sterilization Principle

There are high-temperature dry heat microbial oxidation, protein denaturation , dielectric concentrated cause poisoning. Mainly the destruction of cell protoplasm by oxidation , microbial death, so a certain heating time to kill all microorganisms.

    Dry heat can be used to be able to withstand higher temperatures , but should not be steam penetration , heat sterilization or easily damaged items. Meanwhile dry heat is also used in the pharmaceutical industry in addition to the original method of heat . Because at the same temperature , dry heat microbial killing effect is far less than the saturated steam , dry heat sterilization therefore require higher temperatures or longer sterilization times. Dry heat sterilization is mainly used in the field of various types of laboratory , medical, pharmaceutical , food processing.Feel free to log on http://www.autoclavesale.com/ to see more info.

Heat sterilization Discussion

For now, all sterilization methods available , the heat is an application of the earliest and most reliable results , the most widely used method, which can kill all microorganisms, including bacteria, fungi, protozoa , algae viruses and bacteria more resistant spores, thus sterilized by heat sterilization in industrial importance .

    Thermal methods include moist heat and dry heat . Heat sterilization items in the sterilizer means using pressure steam or other sterilizing medium heat to kill microorganisms , heat sterilization is the most effective and most widely used method . Medicines, liquid, glassware, culture media , dressings, fabric , metal and other equipment when the temperature and humidity do not change or damaged items , can be sterilized by this Act ; rather dry heat sterilization refers to items in dry air heating kill microorganisms reached , usually 160 ~ 170 ℃, 120 min, 170 ~ 180 ℃, 60min, 250 ℃, over 45min under sterile conditions .

Several Parts of Dry Heat Sterilizer

Several Parts of Dry Heat Sterilizer

Major equipment dry heat sterilization dry heat sterilizer , sterilization tunnel system . Dry heat sterilization equipment generally consists of the following important components:

1 ) Heater — it is dry heat sterilization equipment major component , good or bad influence on the sterilization effect is enormous.

2 ) high efficiency filter — in dry heat sterilization equipment , inlet filter should be through efficient ; remove dust material produced internal air circulation system requires efficient filter ; prevent backflow of exhaust pollution can also be installed high efficiency filter devices .

3 ) buffer board , air valve , air conditioner or air baffle — buffer board or air baffle to control the dry heat sterilizer air flow , and more installed in the air intake or exhaust duct or near a heater for the positive control dampers can be installed in the exhaust system to prevent backflow of air pollution in the vicinity of high efficiency filter , positive pressure by controlling the amount of exhaust control can also be used to control air flow regulator inlet and exhaust air volume to maintain a positive pressure .Wanna know more about this?Please link http://www.autoclavesale.com/ to see.

4 ) fan — Dry Heat Sterilizer for air circulation in the affected lot , the wind turbine should be able to measure and adjust .

5 ) the belt (for a continuous process ) — belt speed , dry heat sterilization in the discontinuous transmission system is a very important factor in the sterilization effect .

6 ) Run the chain control system — Dry Heat Sterilizer control system is provided in the chain : door interlock control system ; pressure sensor ; temperature sensing , control, stopping the chain conveyor operation control device so as to ensure that in any case in the case of sterile articles to prevent the sterilization temperature lower than the sterilization occurs through a temperature lower than the design requirements.

7 ) — temperature controller and the recorder in a dry heat sterilization system , the temperature detection sensor , the control , the recording system is the basis of control of sterilization process , the control system must be able to guarantee the sterilization chamber off bacteria can be maintained at a temperature within the range of the set sterilization temperature , the temperature recording system must be detected , the accuracy of the temperature reading sensor system is recorded clearly .

The Main Operation of Dry Heat Sterilizers

The Main Operation of Dry Heat Sterilizers

Dry Heat Sterilizer main operating processes:

1), the items to be sterilized on the inside, to leave gaps when stacked, not allow contact with the wall, steel, close the door.

2), power, located on top of the box to open the vent properly, so that the inside moist air can escape to when the inside temperature reaches 100 ℃ off.Get more info about this on http://www.autoclavesale.com/ .

3), the temperature control knob until the inside temperature reaches the temperature required for the position to observe the temperature is constant. If stable, re-adjust, not stabilized in the vent toggle adjustment knob and keep 140 ~ 160 ℃, 2 ~ 3h.

4), cut off the power after sterilization, to be inside when the temperature dropped to 60 ℃, in order to open the door to remove sterilized items. Meanwhile, the temperature adjustment knob should be adjusted to zero, and open the vent.

The Main Considerations in The Use of Dry Heat Sterilizer

The Main Considerations in The Use of Dry Heat Sterilizer

Because paper and cotton in the above 180 ℃, easily coke fire, so the dry heat sterilization temperature refrain to 180 ℃.

Because of greaseproof paper under high temperature will produce droplets drip onto electric wire easy to fire, so the dry heat sterilization of glassware with greaseproof paper packaging is prohibited.
Due to the sharp drop in temperature will make glassware broken, so the temperature of the oven only dropped to below 60 ℃, in order to open the oven door.
Items should not be put in the oven too much, so as not to affect the air circulation, leaving the temperature on the thermometer indicates allowed, causing temperatures reach above, below temperature, affect the sterilization effect.

Must be heated well before the door closed, or can not enter the heated working condition ( power off).Stay tuned on http://www.autoclavesale.com/ .

Sterilization time must be set before the end of the warm-up program, once inside the insulation stage (ie, the timer starts working) before changing the holding time is invalid.

Operation should always observe the meter is functioning properly, a given temperature, time meets the process requirements.

Must be clean and should be regularly cleaned, ready to clear the inside of residual materials.

Dry Heat Sterilization Effect Monitoring

Dry Heat Sterilization Effect Monitoring

a, chemical monitoring method

Detection Method : The temperature of both the indication temperature of the chemical indicator can indicate the duration of three to five items to be sterilized are placed in the sterilizer and placed on the site most difficult to achieve sterilization . After a sterilization cycle , remove the chemical indicator , according to its color and character to determine whether the change in sterilization conditions .

b, physical detection method ( thermocouple detection )

Detection methods: detection time , a number of multi -point temperature detector probes were placed in the sterilizer layers , the outer points .More info on http://www.autoclavesale.com/ . Shut the door , the wire leads, viewed from the recorder temperature rise and duration. If the temperature ( curve ) shown reaches the preset temperature, the sterilization temperature qualified.

c, bioassays

( 1 ) indicates the strains: Bacillus subtilis (ATCC9372), bacterial film content 5.0 × 105 cfu / piece ~ 5 × 106cfu / film. Its resistance should meet the following conditions: temperature 160 ℃ ± 2 ℃, its D value of 1.3 ~ 1.9min, survival time ≥ 3.9min, killing time ≤ 19min.

( 2 ) detection method: Bacillus subtilis tablets were loaded ( 1 / tube ) in the sterile tubes . Sterilizer door handles with each diagonal , the outer corners of the bacterial film placed two test tubes, test tube cap is placed next to the tube , shut the door , after a sterilization cycle , when the temperature dropped to 80 ℃, after removing the tube capped test tube cap . Under sterile conditions , adding ordinary nutrient broth (5ml / tube ) to 36 ℃ ± 1 ℃ cultured 48h, observe the preliminary results , sterile tube to continue to foster the growth to the seventh day .

( 3 ) Results found: If each chip indicator bacteria inoculated broth tubes are clarified , qualified contractors for the sterilization , if pointed piece of bacteria inoculated broth turbidity , as unqualified for the broth is difficult to determine tube , take 0.1ml inoculated on nutrient agar plates with sterile L stick Tuyun , put 36 ℃ ± 1 ℃ cultured 48h, observing colony morphology and smear staining to determine whether there indicator bacteria growth , if instructions growth of bacteria , as unqualified ; Without indicator bacteria growth , sentenced to sterilization qualified.

Dry Heat Sterilizer Classification

Dry Heat Sterilizer Classification

Dry heat sterilization refers to sterilization in a dry environment (such as a flame or hot dry air) technology. General flame sterilization and dry hot air sterilization.

A flame sterilization method is a method of sterilization using direct flame burning. The method of sterilization rapid, reliable, simple and suitable for flame-resistant material (such as metal, glass and porcelain, etc.) sterilized goods and appliances.

2 dry hot air sterilization method is a high-temperature dry and hot air sterilization methods. The law applies to glass and metal products as well as the gas does not allow heat to penetrate the high-temperature grease (such as oily ointment mechanism for injecting oil, etc.) and high-temperature sterilization powder chemicals. In hot and dry state, due to poor heat penetration, strong heat resistance of microorganisms to be prolonged effect of the high temperature to achieve the purpose of sterilization.

A brief introduction about dry heat sterilization parameters

Dry heat sterilization can be used but should be able to withstand higher temperatures the steam to penetrate , or easily damaged goods moist heat sterilization . Meanwhile dry heat is also used in the pharmaceutical industry in addition to the original method of heat . Ability to evaluate the relative dry heat sterilization , the general death rate can be similar to the units and biological indicators F0 values.Stay tuned on http://www.autoclavesale.com/ .

( A ) D value and the Z value
D is the value at a certain temperature to kill 90% of the microorganism (or a residual ratio of 10% ) the time required for sterilization . Under certain sterilization conditions , different microorganisms have different values ​​of D ; same microbes under different sterilization conditions , D value is not the same . Thus D value with species, environmental and microbial sterilization temperature changes varies .
Z value refers to the sterilization time is reduced to 1/ 10 the desired elevated temperature or at the same time of sterilization , the desired microorganism kill 99% of the increased temperature .
(B ) F value and F0 value
1 . F value

F is at a temperature (T), the sterilization effect produced by a given Z value and the reference temperature in the sterilizing effect (T0) for a given value of Z generated by the same, corresponding to the time of sterilization , units with min . F values ​​commonly used in dry heat sterilization.
2 . F0 value

F0 is a sterilization temperature (T) , the sterilization effect produced at 10 ℃ and sterilization effect produced by the same 121 ℃ considerable time (min). That is , regardless of the temperature change inside the sterilization effect is equivalent to t min F0 minute sterilization at 121 ℃ effect. F0 currently applies only to hot sterilized .

Dry Heat Sterilization PK Moist Heat Sterilization

Dry Heat Sterilization PK Moist Heat Sterilization

Generally heat than dry heat sterilization method is more effective. Moist heat sterilization is the use of hot steam sterilization. At the same temperature, the effect of heat than dry heat sterilization is a good reason:

1, the effect of thermal destruction of the cellular components of more steam. The presence of water molecules contribute to the destruction of the three-dimensional structure of proteins to maintain the hydrogen bonds and other weak bond interactions, more protein denaturation. Protein coagulation temperature is inversely proportional to its water content (specific data available online).

2, hot steam than hot air penetration, can be more effective in killing microorganisms:Stay tuned on http://www.autoclavesale.com/ .

3, the presence of steam latent heat. When the gas into a liquid can release a lot of heat, it can quickly raise the temperature sterilization of objects.

Talk About Ethylene Oxide Sterilization

Talk About Ethylene Oxide Sterilization

Ethylene oxide sterilization was performed using ethylene oxide gas sterilization methods. It is a traditional sterilization method can be applied to clothes sterilization, heat sterilization sterilization intolerance medicines, medical equipment, facilities, and equipment. Ethylene oxide sterilization system, there are important factors that affect the following four mutual restraint sterilizing effect:

1 humidity;

2 temperature;

3 gas concentration;

4 sterilization time.

Because ethylene oxide is a flammable substance can cause burning flame, and because gas decomposition can not cause an explosion, ethylene oxide sterilization should pay great attention to security issues.Stay tuned on http://www.autoclavesale.com/ .

Ethylene oxide has a very strong ability to penetrate and spread, and the mechanism is strong oxidizing primary sterilization, it has a broad spectrum bactericidal strong sterilizing ability characteristics of the propagation of microorganisms, there is a strong effect of killing spores . Although ethylene oxide has a pungent odor and toxicity at high concentrations, it is moderately toxic, generally evaporate quickly, not the surface residual vestiges of the system.